Western blot abreview for Anti-IL-1 beta antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
20 ug/ml poly(I:C)
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
40 second(s)
Bands
Specific: 17 kDa Non-specific: 35 kDa

Abcam user community

Verified customer

Submitted Dec 17 2014

Sign up