Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-IL-1 beta antibody

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde

Other product details

Incubation time
42 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3%NGS in 0.3%Triton X-100-PBS
Dilution
1/250

Secondary antibody

Dilution
1/1000

Additional data

Additional Notes
Rats were perfused with cold PBS, cold 4% PFA, and brains were left in 4% PFA overnight. Brains were then cryoprotected with 30% sucrose solution and stored in -80C. Free floating method was used with 40 micron thick coronal sections. For washes and incubations, 24 well-plate was set on a rotating platform at a speed sufficient enough to keep sections moving in the well. Secondary antibody was incubated for 2 hrs @ RT with the same diluent used for the primary antibody.

Brandon Jun

Verified customer

Submitted Dec 30 2014

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