Immunohistochemistry (Frozen sections) abreview for Anti-IL-1 beta antibody

Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spinal cord)
Permeabilization
Yes - 0.1%triton+0.6%H2O2 in 1XTBST
Specification
spinal cord
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Cy2
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®

Additional data

Additional Notes
40 micron sections

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Submitted Sep 08 2015

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