Flow Cytometry abreview for Anti-IL-1 beta antibody

Excellent
Abreviews
Application
Flow Cytometry
Sample
Rat Cell (Brain)
Permeabilization
Yes - 0.25% Triton X-100
Gating Strategy
single cells population
Specification
Brain
Fixation
Paraformaldehyde

Other product details

Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+5% Donky Serum, overnight, rocking
Dilution
1/100

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor 488 Dye, Invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The samples were incubated with secondary antibodies for 2 hours at room temperature, in the dark with rocking. IgG ab37416 was used (green on the figure).
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback about the use of ab9722 in flow cytometry. We have not tested the antibody in this application before, and we are pleased to see that the antibody is working well for you. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

Ms. Aneta Radziwon-Balicka

Verified customer

Submitted Sep 12 2017

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