Western blot abreview for Anti-IL-1 beta antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse RPE)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount
10 µg
Treatment
high fat diet, inflammation inhibitor, 6 months
Specification
mouse RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/200

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: ECL Rabbit IgG, HRP-linked whole Ab from donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 31, 17.5 kDa Non-specific: 27 kDa

Additional data

Additional Notes
RIPA buffer

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Submitted Mar 04 2019

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