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    il-1-beta-antibody-rm1009-ab283818.pdf

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RecombinantRabMAb

Recombinant Anti-IL-1 beta antibody [RM1009] (ab283818)

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Western blot - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Flow Cytometry - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Flow Cytometry - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunoprecipitation - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Immunoprecipitation - Anti-IL-1 beta antibody [RM1009] (ab283818)
  • Anti-IL-1 beta antibody [RM1009] (ab283818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit recombinant multiclonal [RM1009] to IL-1 beta
  • Suitable for: IHC-P, WB, ICC, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Carrier Free

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Overview

  • Product name

    Anti-IL-1 beta antibody [RM1009]
    See all IL-1 beta primary antibodies
  • Description

    Rabbit recombinant multiclonal [RM1009] to IL-1 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG ; PMA, LPS & BFA treated U-937 ; LPS & BFA treated RAW 264.7 & NR8383 whole cell lysates. IHC-P: Mouse colon carcinoma tissue ; LPS & BFA treated Mouse lung tissue & RAW 264.7. ICC: PMA, LPS & BFA treated U-937 cells ; LPS & BFA treated J774A.1 & RAW 264.7 cells. Flow cyt: PMA, LPS & BFA treated U-937 cells ; LPS & BFA treated J774A.1 cells. IP: PMA, LPS & BFA treated U-937 cells ; LPS & BFA treated J774A.1 & RAW 264.7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Clonality

    Recombinant Multiclonal
  • Clone number

    RM1009
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Obesity
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-IL-1 beta antibody [RM1009] - BSA and Azide free (ab283822)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab283818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000.
ICC
1/50.
IP
1/30.
Flow Cyt
1/500.
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000.
ICC
1/50.
IP
1/30.
Flow Cyt
1/500.

Target

  • Function

    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • Tissue specificity

    Expressed in activated monocytes/macrophages (at protein level).
  • Sequence similarities

    Belongs to the IL-1 family.
  • Post-translational
    modifications

    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • Cellular localization

    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3553 Human
    • Entrez Gene: 16176 Mouse
    • Entrez Gene: 24494 Rat
    • Omim: 147720 Human
    • SwissProt: P01584 Human
    • SwissProt: P10749 Mouse
    • SwissProt: Q63264 Rat
    • Unigene: 126256 Human
    • Unigene: 222830 Mouse
    • Unigene: 9869 Rat
    see all
  • Alternative names

    • Catabolin antibody
    • H1 antibody
    • IFN beta inducing factor antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin 1 beta precursor antibody
    • interleukin 1, beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • Osteoclast activating factor antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Preinterleukin beta antibody
    • Pro interleukin 1 beta antibody
    see all

Images

  • Western blot - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Western blot - Anti-IL-1 beta antibody [RM1009] (ab283818)
    All lanes : Anti-IL-1 beta antibody [RM1009] (ab283818) at 1/1000 dilution

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
    Lane 3 : U-937 treated with 100nM PMA for 2 days, then 1µg/ml LPS for 13h and add 5µg/ml BFA for another 4 h whole cell lysate
    Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 5 : RAW 264.7 treated with 100ng/ml LPS for 4h and add 1µg/ml BFA for another 3 h whole cell lysate
    Lane 6 : NR8383 (Rat lung macrophage (alveolar)) whole cell lysate
    Lane 7 : NR8383 treated with 100ng/ml LPS for 4h and add 1µg/ml BFA for another 3 h whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Observed band size: 17.5,28,31 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Expression of IL-1 beta is induced by LPS treatment. 31-kDa precursor IL-1 beta, 28- and 17.5-kDa proteolytically cleaved IL-1 beta are observed.

    The expression pattern and  molecular weight observed is consistent with what has been described in the literature (PMID: 8446594, 19559631)

    Exposure time: Lane 1-3: 3 min ; Lane 4-7: 70 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Immunohistochemical analysis of paraffin-embedded Mouse colon carcinom tissue labelling IL-1 beta with ab283818 at 1/500 (1.058 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on the lamina propria in mouse colon carcinoma. The section was incubated with ab283818 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Immunohistochemical analysis of paraffin-embedded RAW 264.7 cells labelling IL-1 beta with ab283818 at 1/500 (1.058 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on RAW 264.7 cells treated with LPS (100ng/ml for 4+3 hours) added BFA (1µg/ml for 3 hours) (Image A) and no staining on control RAW 264.7 (Image B). The section was incubated with ab283818 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling IL-1 beta with ab283818 at 1/500 (1.058 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on mouse lung treated with LPS (1ug/ml for 16 hours) and BFA (1ug/ml for 16h hours) (Image A) and no staining on control mouse lung was observed (Image B). The section was incubated with ab283818 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling IL-1 beta with ab283818 at 1/500 (1.058 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). The section was incubated with ab283818 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: no staining on mouse colon.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling IL-1 beta with ab283818 at 1/500 (1.058 ug/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). The section was incubated with ab283818 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: no staining on mouse spleen.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-937 cells labelling IL-1 beta with ab283818 at 1/50 (10.58 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in U-937 cells treated with Phorbol-12-myristate-13-acetate (100 nM) for 2 days, then replaced it with Lipopolysaccharides (1 µg/ml) for 13 h, with the addition of Brefeldin A (5 µg/ml) for the last 4 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 cells labelling IL-1 beta with ab283818 at 1/50 (10.58 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in J774A.1 cells treated with Lipopolysaccharides (100 ng/ml) for 4h, with the addition of brefeldin A (1 ug/ml) for the last 3 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 cells labelling IL-1 beta with ab283818 at 1/50 (10.58 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and nuclear staining in RAW 264.7 cells transfected with Lipopolysaccharides (100 ng/ml) for 4h, with the addition of brefeldin A (1 ug/ml) for the last 3 hours. (PMID:18490713, PMID:18939951). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Flow Cytometry - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Flow Cytometry - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-937 (Human histiocytic lymphoma monocyte) treated with 100nM PMA for 2days then 1µg/ml LPS treated for 13h and add 5µg/ml BFA for another 4h (Red) / U-937 treated with 100nM PMA for 2days (Green) cells labelling IL-1 beta with ab283818 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Flow Cytometry - Anti-IL-1 beta antibody [RM1009] (ab283818)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) treated with 100ng/ml LPS treated for 4h and add 1µg/ml BFA for another 3h (Red) / Untreated control (Green) cells labelling IL-1 beta with ab283818 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunoprecipitation - Anti-IL-1 beta antibody [RM1009] (ab283818)

    IL-1 beta was immunoprecipitated from 0.35 mg U-937 (Human histiocytic lymphoma monocyte) treated with 100nM PMA for 2 days, then 1µg/ml LPS for 13h and add 5µg/ml BFA for another 4 h, whole cell lysate 10ug with ab283818 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283818 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: U-937 (Human histiocytic lymphoma monocyte) treated with 100nM PMA for 2 days, then 1µg/ml LPS for 13h and add 5µg/ml BFA for another 4 h, whole cell lysate 10ug

    Lane 2: ab283818 IP in U-937 treated with 100nM PMA for 2 days, then 1µg/ml LPS for 13h and add 5µg/ml BFA for another 4 h, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab283818 in U-937 treated with 100nM PMA for 2 days, then 1µg/ml LPS for 13h and add 5µg/ml BFA for another 4 h, whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-IL-1 beta antibody [RM1009] (ab283818)
    Immunoprecipitation - Anti-IL-1 beta antibody [RM1009] (ab283818)

    IL-1 beta was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 4h and add 1ug/ml BFA for another 3 h whole cell lysate 10ug with ab283818 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283818 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 4h and add 1ug/ml BFA for another 3 h whole cell lysate 10ug

    Lane 2: ab283818 IP in RAW 264.7 treated with 100ng/ml LPS for 4h and add 1ug/ml BFA for another 3 h whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab283818 in RAW 264.7 treated with 100ng/ml LPS for 4h and add 1ug/ml BFA for another 3 h whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

     

  • Anti-IL-1 beta antibody [RM1009] (ab283818)
    Anti-IL-1 beta antibody [RM1009] (ab283818)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab283818? Please let us know so that we can cite the reference in this datasheet.

ab283818 has not yet been referenced specifically in any publications.

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