Overview

  • Product name

    Anti-IL-10 antibody [4A7-25-17]
    See all IL-10 primary antibodies
  • Description

    Mouse monoclonal [4A7-25-17] to IL-10
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IL-10.

  • Positive control

    • Human pancreas adenocarcinoma tissue; recombinant Human IL-10 protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab134742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 20 kDa.

Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
We recommend 3% Nonfat Dry Milk in diH2O as a blocking solution

IHC-P Use a concentration of 5 µg/ml.

Customer feedback suggests heat-mediated antigen retrieval with Tris-EDTA pH9.0 is effective.

Sandwich ELISA Use a concentration of 8 - 10 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL-10 (Biotin) (ab84031).

8.0-10.0 µg/ml of this antibody will detect at least 1000 pg/well of recombinant Human IL-10. As the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.

Target

  • Function

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human pancreas adenocarcinoma tissue labelling IL-10 with ab134742 antibody at a concentration of 5 µg/ml.

  • Immunohistochemical analysis of paraffin embedded Human pancreas adenocarcinoma tissue labelling IL-10 with ab134742 antibody at a concentration of 5 µg/ml.

  • Immunohistochemical analysis of paraffin embedded Human pancreas adenocarcinoma tissue labelling IL-10 with ab134742 antibody at a concentration of 5 µg/ml.

  • Sandwich ELISA of recombinant IL-10 (assuming 100µl/well), a concentration of 8.0-10.0 µg/ml of ab134742 will detect at least 1000 pg/well of recombinant Human IL-10 when used with an appropriate detection antibody.

References

This product has been referenced in:

  • Bing X  et al. Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis. Can J Gastroenterol Hepatol 2018:5760849 (2018). Read more (PubMed: 29765931) »
  • Sauter MM & Brandt CR Primate neural retina upregulates IL-6 and IL-10 in response to a herpes simplex vector suggesting the presence of a pro-/anti-inflammatory axis. Exp Eye Res 148:12-23 (2016). Read more (PubMed: 27170050) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-Edta pH 10
Permeabilization
No
Specification
Lymph node
Blocking step
Protein Block serum free from Dako as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 03 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Muller Cell Line MIO-M1)
Permeabilization
Yes - 0.1% TritonX-100
Specification
Muller Cell Line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 01 2016

Application
Western blot
Sample
Cynomolgus monkey Tissue lysate - whole (retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
50 µg
Specification
retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 17 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Cell (RPE cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TrisEDTA pH 9.0
Permeabilization
No
Specification
RPE cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Oct 20 2015

Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil

Mr. Ali Hosseini

Verified customer

Submitted Oct 27 2014

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