Product nameAnti-IL-10 antibody
See all IL-10 primary antibodies
DescriptionRabbit polyclonal to IL-10
Tested applicationsSuitable for: WB, IHC-Fr, ELISA, IHC-P, ICC/IF, Neutralising, RIAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IL-10.
- WB: 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC)(PBMC stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS); IL-1 stimulated IL- 10 production from fibroblasts, recombinant protein
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituent: Whole serum
Purification notesab34843 antiserum has been sterile filtered and heated to 56°C for 30 minutes.
Our Abpromise guarantee covers the use of ab34843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.
|ELISA||1/20000 - 1/100000.
(as detection antibody, using a monoclonal antibody for capture).
|ICC/IF||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Sequence similaritiesBelongs to the IL-10 family.
- Information by UniProt
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
ab34843 staining IL-10 in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 20 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
Immunofluorescence analysis of Human peripheral blood mononuclear cells, staining IL-10 with ab34843.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/250) was used as the secondary antibody.
This product has been referenced in:
- Castrogiovanni P et al. Moderate Physical Activity as a Prevention Method for Knee Osteoarthritis and the Role of Synoviocytes as Biological Key. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30691048) »
- Vartina E et al. Inflammatory cytokines and antimicrobial peptides in acquired heart diseases. Histol Histopathol N/A:18091 (2019). Read more (PubMed: 30734265) »