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    il-10-antibody-ab34843.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-10 antibody (ab34843)

  • Datasheet
  • SDS
Reviews (13)Q&A (16)References (48)

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Abpromise

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Western blot - Anti-IL-10 antibody (ab34843)
  • Western blot - Anti-IL-10 antibody (ab34843)

Key features and details

  • Rabbit polyclonal to IL-10
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-IL-10 antibody [EP1115Y] (ab52909)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-IL-10 antibody
    See all IL-10 primary antibodies
  • Description

    Rabbit polyclonal to IL-10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IL-10.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human IL-10 protein (Active) (ab259402)

Applications

Our Abpromise guarantee covers the use of ab34843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 21 kDa.

In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.

Target

  • Function

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P22301 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3586 Human
    • Omim: 124092 Human
    • SwissProt: P22301 Human
    • Unigene: 193717 Human
    • Alternative names

      • CSIF antibody
      • Cytokine synthesis inhibitory factor antibody
      • GVHDS antibody
      • IL 10 antibody
      • IL-10 antibody
      • IL10 antibody
      • IL10_HUMAN antibody
      • IL10A antibody
      • Interleukin 10 antibody
      • Interleukin-10 antibody
      • MGC126450 antibody
      • MGC126451 antibody
      • T-cell growth inhibitory factor antibody
      • TGIF antibody
      see all

    Images

    • Western blot - Anti-IL-10 antibody (ab34843)
      Western blot - Anti-IL-10 antibody (ab34843)
      Anti-IL-10 antibody (ab34843) at 1/200 dilution + recombinant IL-10 protein

      Secondary
      Goat anti-rabbit at 1/20000 dilution

      Predicted band size: 21 kDa



       
       
      4-20% reducing gel.
      Membrane was blocked in 3% BSA-TBS for 1 hour 4°C.
      Dilution buffer: 3% BSA-TBS.
      Incubated O/N at 4°C.
    • Western blot - Anti-IL-10 antibody (ab34843)
      Western blot - Anti-IL-10 antibody (ab34843)
      Anti-IL-10 antibody (ab34843) at 1/500 dilution + recombinant IL-10 protein, 50ng

      Secondary
      Peroxidase conjugated secondary at 1/40000 dilution

      Predicted band size: 21 kDa

    Protocols

    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (48)

    Publishing research using ab34843? Please let us know so that we can cite the reference in this datasheet.

    ab34843 has been referenced in 48 publications.

    • Duan X & Qin G Notch inhibitor mitigates renal ischemia-reperfusion injury in diabetic rats. Mol Med Rep 21:583-588 (2020). PubMed: 31974606
    • Shigeoka M  et al. CD163+ macrophages infiltration correlates with the immunosuppressive cytokine interleukin 10 expression in tongue leukoplakia. Clin Exp Dent Res 5:627-637 (2019). PubMed: 31890299
    • Chen S  et al. Eluted 25-hydroxyvitamin D3 from radially aligned nanofiber scaffolds enhances cathelicidin production while reducing inflammatory response in human immune system-engrafted mice. Acta Biomater 97:187-199 (2019). PubMed: 31386930
    • Li M  et al. Losmapimod Protected Epileptic Rats From Hippocampal Neuron Damage Through Inhibition of the MAPK Pathway. Front Pharmacol 10:625 (2019). PubMed: 31231220
    • Banerjee J  et al. Delivery of silver sulfadiazine and adipose derived stem cells using fibrin hydrogel improves infected burn wound regeneration. PLoS One 14:e0217965 (2019). PubMed: 31194776
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 29 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-10 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (spinal cord transversal section)
    Permeabilization
    Yes - 0,01% Triton-X 100
    Specification
    spinal cord transversal section
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Tamas Bellak

    Verified customer

    Submitted Dec 01 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica BOND ER2
    Permeabilization
    No
    Specification
    tonsil
    Fixative
    Formaldehyde
    Read More

    Mr. Masahiro Hirata

    Verified customer

    Submitted Sep 09 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (CT26 tumor taken out Balb/C mice)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Ph6
    Permeabilization
    No
    Specification
    CT26 tumor taken out Balb/C mice
    Blocking step
    (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 15 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (MDA-MB-231 (ATCC® HTB-26™) tumor taken out of Balb)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    MDA-MB-231 (ATCC® HTB-26™) tumor taken out of Balb
    Blocking step
    Innovex Background Buster as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 20 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue (intestine)
    Specification
    intestine
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 05 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-10 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human skin)
    Permeabilization
    Yes - 0.5% Triton TBS Ca/Azide
    Specification
    Human skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 18 2018

    Western blot abreview for Anti-IL-10 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cynomolgus monkey Tissue lysate - whole (retina)
    Gel Running Conditions
    Reduced Denaturing (4-15% gel)
    Loading amount
    50 µg
    Specification
    retina
    Blocking step
    Milk as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 18 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (Retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 02 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-10 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human head & neck squamous cell carcinoma)
    Permeabilization
    Yes - Triton X-100 (0.1%) for 5 min
    Specification
    Human head & neck squamous cell carcinoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 29 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-10 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human peripheral blood mononuclear cells)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS, 3min
    Specification
    Human peripheral blood mononuclear cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 28 2013

    1-10 of 29 Abreviews or Q&A

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