Anti-IL-10 antibody (ab34843)
Key features and details
- Rabbit polyclonal to IL-10
- Suitable for: RIA, IHC-Fr, Neutralising, WB, IHC-P, ICC/IF, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IL-10 antibody
See all IL-10 primary antibodies -
Description
Rabbit polyclonal to IL-10 -
Host species
Rabbit -
Tested applications
Suitable for: RIA, IHC-Fr, Neutralising, WB, IHC-P, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Full length native protein (purified) corresponding to Human IL-10.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| RIA |
Use at an assay dependent concentration.
|
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| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| Neutralising |
Use at an assay dependent concentration.
|
|
| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
| IHC-P | (8) |
Use at an assay dependent concentration.
|
| ICC/IF | (3) |
Use at an assay dependent concentration.
|
| ELISA |
Use at an assay dependent concentration.
(as detection antibody, using a monoclonal antibody for capture). |
| Notes |
|---|
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RIA
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Neutralising
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. (as detection antibody, using a monoclonal antibody for capture). |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
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Western blot - Anti-IL-10 antibody (ab34843)Anti-IL-10 antibody (ab34843) at 1/200 dilution + recombinant IL-10 protein
Secondary
Goat anti-rabbit at 1/20000 dilution
Predicted band size: 21 kDa
4-20% reducing gel.Membrane was blocked in 3% BSA-TBS for 1 hour 4°C.Dilution buffer: 3% BSA-TBS.Incubated O/N at 4°C. -
Western blot - Anti-IL-10 antibody (ab34843)Anti-IL-10 antibody (ab34843) at 1/500 dilution + recombinant IL-10 protein, 50ng
Secondary
Peroxidase conjugated secondary at 1/40000 dilution
Predicted band size: 21 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (70)
ab34843 has been referenced in 70 publications.
- Paschalidi P et al. The role of M1 and M2 macrophage polarization in progression of medication-related osteonecrosis of the jaw. Clin Oral Investig 25:2845-2857 (2021). PubMed: 32964311
- Pichler R et al. Expression of ADAM Proteases in Bladder Cancer Patients with BCG Failure: A Pilot Study. J Clin Med 10:N/A (2021). PubMed: 33672843
- Cizkova K et al. Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells. Acta Histochem Cytochem 54:21-29 (2021). PubMed: 33731967
- Wilkinson H et al. PAR-1 signaling on macrophages is required for effective in vivo delayed-type hypersensitivity responses. iScience 24:101981 (2021). PubMed: 33458623
- Montanaro M et al. The Paradox Effect of Calcification in Carotid Atherosclerosis: Microcalcification is Correlated with Plaque Instability. Int J Mol Sci 22:N/A (2021). PubMed: 33401449