For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    il-10-antibody-ab9969.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Cytokines Interleukins
Share by email

Anti-IL-10 antibody (ab9969)

  • Datasheet
  • SDS
Reviews (3)Q&A (7)References (66)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Sandwich ELISA - Anti-IL-10 antibody (ab9969)

    Key features and details

    • Rabbit polyclonal to IL-10
    • Suitable for: ELISA, WB, ICC/IF, Sandwich ELISA
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-CD16 antibody [EPR20537] (ab211151)
    ELISA
    Product image
    Mouse IL-22 ELISA Kit (ab223857)
    ELISA
    Product image
    Mouse IL-10 ELISA Kit (ab255729)

    View more associated products

    Overview

    • Product name

      Anti-IL-10 antibody
      See all IL-10 primary antibodies
    • Description

      Rabbit polyclonal to IL-10
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ELISA, WB, ICC/IF, Sandwich ELISAmore details
    • Species reactivity

      Reacts with: Mouse, Rat
    • Immunogen

      Recombinant fragment corresponding to Rat IL-10 aa 19-178.
      (Peptide available as ab9970)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      No preservative, sterile filtered
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Light chain type

      unknown
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Recombinant rat IL-10 protein (Active) (ab9970)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human IL-10 protein (Active) (ab259402)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9969 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. Can be blocked with Recombinant rat IL-10 protein (Active) (ab9970).
    ICC/IF (1)
    Use at an assay dependent concentration.
    Sandwich ELISA
    Use a concentration of 0.5 - 2 µg/ml.
    Notes
    ELISA
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. Can be blocked with Recombinant rat IL-10 protein (Active) (ab9970).
    ICC/IF
    Use at an assay dependent concentration.
    Sandwich ELISA
    Use a concentration of 0.5 - 2 µg/ml.

    Target

    • Function

      Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
    • Tissue specificity

      Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
    • Sequence similarities

      Belongs to the IL-10 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P22301 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 16153 Mouse
      • Entrez Gene: 25325 Rat
      • SwissProt: P18893 Mouse
      • SwissProt: P29456 Rat
      • Unigene: 874 Mouse
      • Unigene: 9868 Rat
      • Alternative names

        • CSIF antibody
        • Cytokine synthesis inhibitory factor antibody
        • GVHDS antibody
        • IL 10 antibody
        • IL-10 antibody
        • IL10 antibody
        • IL10_HUMAN antibody
        • IL10A antibody
        • Interleukin 10 antibody
        • Interleukin-10 antibody
        • MGC126450 antibody
        • MGC126451 antibody
        • T-cell growth inhibitory factor antibody
        • TGIF antibody
        see all

      Images

      • Sandwich ELISA - Anti-IL-10 antibody (ab9969)
        Sandwich ELISA - Anti-IL-10 antibody (ab9969)

        Sandwich ELISA ab9969. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (66)

      Publishing research using ab9969? Please let us know so that we can cite the reference in this datasheet.

      ab9969 has been referenced in 66 publications.

      • Ni XQ  et al. Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway. Aging (Albany NY) 13:5164-5184 (2021). PubMed: 33535178
      • Han Z  et al. Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis. Aging (Albany NY) 13:5087-5103 (2021). PubMed: 33535180
      • Zhu HS  et al. Prior transfusion of umbilical cord mesenchymal stem cells can effectively alleviate symptoms of motion sickness in mice through interleukin 10 secretion. World J Stem Cells 13:177-192 (2021). PubMed: 33708346
      • Adams JA  et al. Whole body periodic acceleration (pGz) improves endotoxin induced cardiomyocyte contractile dysfunction and attenuates the inflammatory response in mice. Heliyon 7:e06444 (2021). PubMed: 33748496
      • Cui H & Zhang Q Dexmedetomidine ameliorates lipopolysaccharide-induced acute lung injury by inhibiting the PI3K/Akt/FoxO1 signaling pathway. J Anesth 35:394-404 (2021). PubMed: 33821300
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-10 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (immortalised bone marrow-derived macrophage)
      Permeabilization
      Yes - .01% Triton X100
      Specification
      immortalised bone marrow-derived macrophage
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      DR. James Harris

      Verified customer

      Submitted Jan 31 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2022 Abcam plc. All rights reserved.