Anti-IL-10 antibody (ab9969)
Key features and details
- Rabbit polyclonal to IL-10
- Suitable for: ELISA, WB, ICC/IF, Sandwich ELISA
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-IL-10 antibody
See all IL-10 primary antibodies -
Description
Rabbit polyclonal to IL-10 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, ICC/IF, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Rat IL-10 aa 19-178.
(Peptide available asab9970) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Can be blocked with Recombinant rat IL-10 protein (Active) (ab9970).
|
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Can be blocked with Recombinant rat IL-10 protein (Active) (ab9970). |
|
ICC/IF
Use at an assay dependent concentration. |
|
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. |
Target
-
Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 16153 Mouse
- Entrez Gene: 25325 Rat
- SwissProt: P18893 Mouse
- SwissProt: P29456 Rat
- Unigene: 874 Mouse
- Unigene: 9868 Rat
-
Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
-
Sandwich ELISA - Anti-IL-10 antibody (ab9969)
Sandwich ELISA ab9969. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (66)
ab9969 has been referenced in 66 publications.
- Ni XQ et al. Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway. Aging (Albany NY) 13:5164-5184 (2021). PubMed: 33535178
- Han Z et al. Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis. Aging (Albany NY) 13:5087-5103 (2021). PubMed: 33535180
- Zhu HS et al. Prior transfusion of umbilical cord mesenchymal stem cells can effectively alleviate symptoms of motion sickness in mice through interleukin 10 secretion. World J Stem Cells 13:177-192 (2021). PubMed: 33708346
- Adams JA et al. Whole body periodic acceleration (pGz) improves endotoxin induced cardiomyocyte contractile dysfunction and attenuates the inflammatory response in mice. Heliyon 7:e06444 (2021). PubMed: 33748496
- Cui H & Zhang Q Dexmedetomidine ameliorates lipopolysaccharide-induced acute lung injury by inhibiting the PI3K/Akt/FoxO1 signaling pathway. J Anesth 35:394-404 (2021). PubMed: 33821300