Anti-IL-10 antibody [B-S10] - BSA and Azide free (ab47222)
Key features and details
- Mouse monoclonal [B-S10] to IL-10 - BSA and Azide free
- Suitable for: Functional Studies
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-IL-10 antibody [B-S10] - BSA and Azide free
See all IL-10 primary antibodies -
Description
Mouse monoclonal [B-S10] to IL-10 - BSA and Azide free -
Host species
Mouse -
Specificity
This antibody recognizes both natural and recombinant IL-10.
Blocks IL-10 induced inhibition of IL-6 synthesis on LPS activated monocytes.
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Tested applications
Suitable for: Functional Studiesmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IL-10.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
Carrier free
Yes -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
This antibody is sterile filtered through 0.22 µm and treated to remove endotoxins. It is carrier and preservative free. -
Clonality
Monoclonal -
Clone number
B-S10 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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Functional Studies
Use at an assay dependent concentration. |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab47222 has been referenced in 1 publication.
- Bernatowicz PL et al. Synergistic effect of Dermatophagoides pteronyssinus allergens and dexamethasone on the expression of CD163 by peripheral blood mononuclear cells. Immunobiology 224:497-501 (2019). PubMed: 31133346