FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271261 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Sequence similaritiesBelongs to the IL-10 family.
- Information by UniProt
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
Western blot analysis of varying levels of Recombinant human IL-10 protein using ab271261 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Mouse mIL-10 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271261. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/ well of recombinant mIL-10 protein.
ab271261 has not yet been referenced specifically in any publications.