Overview

  • Product name

    Anti-IL-10 antibody [JES5-2A5]
    See all IL-10 primary antibodies
  • Description

    Rat monoclonal [JES5-2A5] to IL-10
  • Host species

    Rat
  • Specificity

    ab189392 recognizes mouse IL-10. IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells.
  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse IL-10.
    Database link: P18893

  • Positive control

    • Mouse small intestine and spleen tissues. Recombinant mouse IL-10.

Properties

Applications

Our Abpromise guarantee covers the use of ab189392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

Use 10 µl of the suggested working dilution to label 1x106 cells in 100 µl. Membrane permeabilization is required for this application.

 

 

 

 

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, labeling IL-10 using ab189392 at 10 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, labeling IL-10 using ab189392 at 10 µg/ml.

  • Anti-IL-10 antibody [JES5-2A5] (ab189392) at 1/1000 dilution + recombinant mouse IL-10

    Secondary
    F(ab)2 rabbit anti-rat IgG:HRP

    Developed using the ECL technique.

    Predicted band size: 21 kDa

References

This product has been referenced in:

  • Kleinerman ES  et al. Aerosol Gemcitabine after Amputation Inhibits Osteosarcoma Lung Metastases but Not Wound Healing. Sarcoma 2018:3143096 (2018). Read more (PubMed: 29610563) »
  • Yu Y  et al. IPSC-MSC inhibition assessment in Raw 264.7 cells following oxygen and glucose deprivation reveals a distinct function for cardiopulmonary resuscitation. Mol Med Rep 17:8212-8220 (2018). Read more (PubMed: 29658608) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Paw)
Antigen retrieval step
None
Permeabilization
No
Specification
Paw
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 05 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Retina and Colon)
Antigen retrieval step
None
Permeabilization
No
Specification
Retina and Colon
Blocking step
Serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
Gluteraldehyde

Premier Laboratory

Verified customer

Submitted Apr 05 2016

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