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  1. Link

    il-10-antibody-jes5-2a5-ab189392.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-10 antibody [JES5-2A5] (ab189392)

  • Datasheet
Reviews (2) Submit a question References (23)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)
  • Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392)

Key features and details

  • Rat monoclonal [JES5-2A5] to IL-10
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG1

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Overview

  • Product name

    Anti-IL-10 antibody [JES5-2A5]
    See all IL-10 primary antibodies
  • Description

    Rat monoclonal [JES5-2A5] to IL-10
  • Host species

    Rat
  • Specificity

    ab189392 recognizes mouse IL-10. IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse IL-10.
    Database link: P18893

  • Positive control

    • Mouse small intestine and spleen tissues. Recombinant mouse IL-10.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    JES5-2A5
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-IL-10 antibody [JES5-2A5] (ab33471)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
  • Recombinant Protein

    • Recombinant human IL-10 protein (Active) (ab259402)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab189392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 21 kDa.
IHC-P (2)
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000. Predicted molecular weight: 21 kDa.
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P22301 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 16153 Mouse
    • SwissProt: P18893 Mouse
    • Unigene: 874 Mouse
    • Alternative names

      • CSIF antibody
      • Cytokine synthesis inhibitory factor antibody
      • GVHDS antibody
      • IL 10 antibody
      • IL-10 antibody
      • IL10 antibody
      • IL10_HUMAN antibody
      • IL10A antibody
      • Interleukin 10 antibody
      • Interleukin-10 antibody
      • MGC126450 antibody
      • MGC126451 antibody
      • T-cell growth inhibitory factor antibody
      • TGIF antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, labeling IL-10 using ab189392 at 10 µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, labeling IL-10 using ab189392 at 10 µg/ml.

    • Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392)
      Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392)
      Anti-IL-10 antibody [JES5-2A5] (ab189392) at 1/1000 dilution + recombinant mouse IL-10

      Secondary
      F(ab)2 rabbit anti-rat IgG:HRP

      Developed using the ECL technique.

      Predicted band size: 21 kDa

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (23)

    Publishing research using ab189392? Please let us know so that we can cite the reference in this datasheet.

    ab189392 has been referenced in 23 publications.

    • Wei Y  et al. Acacetin ameliorates insulin resistance in obesity mice through regulating Treg/Th17 balance via MiR-23b-3p/NEU1 Axis. BMC Endocr Disord 21:57 (2021). PubMed: 33781239
    • Chen SJ  et al. Targeting lysosomal cysteine protease cathepsin S reveals immunomodulatory therapeutic strategy for oxaliplatin-induced peripheral neuropathy. Theranostics 11:4672-4687 (2021). PubMed: 33754020
    • Sari RP  et al. The Effect of Anadara granosa Shell's-Stichopus hermanni Scaffold on CD44 and IL-10 Expression to Decrease Osteoclasts in Socket Healing. Eur J Dent 15:228-235 (2021). PubMed: 33511601
    • Fernández-Albarral JA  et al. Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of Glaucoma. Int J Mol Sci 22:N/A (2021). PubMed: 33669765
    • Rojas P  et al. Retinal Ganglion Cell Loss and Microglial Activation in a SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis. Int J Mol Sci 22:N/A (2021). PubMed: 33562231
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody [JES5-2A5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Paw)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Paw
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 05 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody [JES5-2A5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Retina and Colon)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Retina and Colon
    Blocking step
    Serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative
    Gluteraldehyde
    Read More

    Premier Laboratory

    Verified customer

    Submitted Apr 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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