Anti-IL-10 antibody [JES5-2A5] (ab189392)
Key features and details
- Rat monoclonal [JES5-2A5] to IL-10
- Suitable for: IHC-Fr, WB, IHC-P, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-IL-10 antibody [JES5-2A5]
See all IL-10 primary antibodies -
Description
Rat monoclonal [JES5-2A5] to IL-10 -
Host species
Rat -
Specificity
ab189392 recognizes mouse IL-10. IL-10 is expressed by Ly-1 (B-1) B cells that express CD5 (Ly-1) and a subset of lectin stimulated T cells. -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse IL-10.
Database link: P18893 -
Positive control
- Mouse small intestine and spleen tissues. Recombinant mouse IL-10.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JES5-2A5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab189392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/1000. Predicted molecular weight: 21 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. Use 10 µl of the suggested working dilution to label 1x106 cells in 100 µl. Membrane permeabilization is required for this application.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16153 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)](https://www.abcam.com/ps/products/189/ab189392/Images/ab189392-224167-anti-il-10-antibody-jes5-2a5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue, labeling IL-10 using ab189392 at 10 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)](https://www.abcam.com/ps/products/189/ab189392/Images/ab189392-224168-anti-il-10-antibody-jes5-2a5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody [JES5-2A5] (ab189392)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse spleen tissue, labeling IL-10 using ab189392 at 10 µg/ml.
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![Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392)](https://www.abcam.com/ps/products/189/ab189392/Images/ab189392-224169-anti-il-10-antibody-jes5-2a5-western-blot.jpg)
Western blot - Anti-IL-10 antibody [JES5-2A5] (ab189392)Anti-IL-10 antibody [JES5-2A5] (ab189392) at 1/1000 dilution + recombinant mouse IL-10
Secondary
F(ab)2 rabbit anti-rat IgG:HRP
Developed using the ECL technique.
Predicted band size: 21 kDa
Protocols
Datasheets and documents
References
ab189392 has been referenced in 11 publications.
- Carvalho TPV et al. Does physical exercise influence in the development of neuroeschistosomiasis? Brain Res Bull 152:311-322 (2019). PubMed: 31377443
- Ren C et al. An effective and biocompatible polyethylenimine based vaginal suppository for gene delivery. Nanomedicine 20:101994 (2019). PubMed: 31028885
- Patra V et al. Skin Microbiome Modulates the Effect of Ultraviolet Radiation on Cellular Response and Immune Function. iScience 15:211-222 (2019). PubMed: 31079025
- Bhattacharjee S et al. Concomitant Infection of S. mansoni and H. pylori Promotes Promiscuity of Antigen-Experienced Cells and Primes the Liver for a Lower Fibrotic Response. Cell Rep 28:231-244.e5 (2019). PubMed: 31269443
- Kleinerman ES et al. Aerosol Gemcitabine after Amputation Inhibits Osteosarcoma Lung Metastases but Not Wound Healing. Sarcoma 2018:3143096 (2018). PubMed: 29610563
