Anti-IL-10 antibody [JES5-2A5] (ab33471)

Thank you for contacting us.

I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around aa13-aa70 of mouse IL-10 (http://www.uniprot.org/uniprot/P18893).

The peptide from which ab9969 was created has 78% identity. Generally we do not expect an antibody to recognize a target if the homology is below 85% but this product is polyclonal and as such may be able to recognize human IL-10 in some lots.



The immunogen used to generate ab33471 is E coli-expressed recombinant mouse IL-10. We have not mapped the epitope of this antibody so we do not know exactly where the antibody will bind. .However this is a monoclonal product and as stated above the homology between IL-1o mouse and human is below the threshold for antibody recognition. As such this may be an appropriate choice but we have not tested in-house for cross reactivity of this antibody andcannot guarantee that it will not recognize human IL-10.



The same statements above can be repeated for ab25073. However as this is a mouse monoclonal and you wish to work in mouse tissue we would highly recommend use of theMouse on Mouse Polymer IHC Kit ab127055.



AB33471 and ab9969 would also qualify for enrollment in our AbTrial testing program. For UNTESTED applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here:https://www.abcam.com/AbTrial

I have included links to each of these products below:

AB9969:https://www.abcam.com/il10-antibody-ab9969.html

AB33471:https://www.abcam.com/il10-antibody-jes5-2a5-ab33471.html

AB25073:https://www.abcam.com/il10-antibody-2g101h7-ab25073.html

Mouse on Mouse Kit:https://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

I hope this information is helpful to you. If you are interested in testing one of these products in IHC-P please let me know. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for contacting us.

We haven't tested this antibody in paraffin embedded tissue sections (IHC-P), only frozen sections from mouse. However, IL10 is produced and secreted by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. Any tissue that has a high concentration of these cells would serve as a good positive control. Other IL10 antibodies have been used to stain colon and hippocampus, so theoretically the IL10 protein should be found in these tissues regardless of which antibody is used.

https://www.abcam.com/index.html?datasheet=34843&tab=abreviews&intabreviewid=16485

https://www.abcam.com/IL10-antibody-2G101H7-ab25073.html

And although it is for human IL10, you should check out the IHC staining from the Human Protein Atlas for IL10.

http://www.proteinatlas.org/ENSG00000136634/normal

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

We typically load 10 - 20 nanograms of purified recombinant protein in gels for western blots. I have contacted the laboratory to find out the exact amount that was used for the blot on the datasheet but I expect it will be within that range.

Thank you for contacting us.

We recommend ab18412.

Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply and for sending your protocol information and image. We do guarantee ab33471 to work in Western blot with mouse lysates, so we are happy to issue you a refund for this purchase. I have requested the refund to our accounting department, and this purchase will be refunded back to your credit card. If you have any questions about the status of this refund, please contact us.credits@abcam.com and refer to credit note ***. Please let me know if you have any questions, or if there is anything else that we can do for you.