Key features and details
- Rabbit polyclonal to IL-10RA
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-IL-10RA antibody
See all IL-10RA primary antibodies
DescriptionRabbit polyclonal to IL-10RA
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human IL-10RA aa 22-230.
HGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWNS ISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVD EVTLTVGSVNLEIHNGFILGKIQLPRPKMAPANDTYESIFSHFREYEIAI RKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVASRSNKGMWSKE ECISLTRQY
Database link: Q13651
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab225820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 72 kDa (predicted molecular weight: 63 kDa).|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.|
FunctionReceptor for IL10; binds IL10 with a high affinity.
Tissue specificitySpleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels.
Involvement in diseaseInflammatory bowel disease 28
Sequence similaritiesBelongs to the type II cytokine receptor family.
- Information by UniProt
- CD210 antibody
- CDw210a antibody
- CDw210a antigen antibody
All lanes : Anti-IL-10RA antibody (ab225820) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) extract
Lane 3 : Mouse brain extract
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
Blocking buffer: 3% non-fat dry milk in TBST.
U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for IL-10RA (green) using ab225820 at 1/50 dilution in ICC/IF. DAPI (blue) for nuclear staining.
Paraffin-embedded rat brain tissue stained for IL-10RA with ab225820 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for IL-10RA with ab225820 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded mouse brain tissue stained for IL-10RA with ab225820 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225820 has been referenced in 1 publication.
- Dai WJ et al. Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision Pain. Evid Based Complement Alternat Med 2019:8413576 (2019). PubMed: 31885668