Anti-IL-10RA antibody (ab225820)
Key features and details
- Rabbit polyclonal to IL-10RA
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-IL-10RA antibody
See all IL-10RA primary antibodies -
Description
Rabbit polyclonal to IL-10RA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Human IL-10RA aa 1-250.
Database link: Q13651 -
Positive control
- WB: Mouse brain tissue lysate, MCF7 and K562 cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Detects a band of approximately 72 kDa (predicted molecular weight: 63 kDa).
|
Notes |
---|
WB
1/500 - 1/2000. Detects a band of approximately 72 kDa (predicted molecular weight: 63 kDa). |
Target
-
Function
Receptor for IL10; binds IL10 with a high affinity. -
Tissue specificity
Spleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels. -
Involvement in disease
Inflammatory bowel disease 28 -
Sequence similarities
Belongs to the type II cytokine receptor family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3587 Human
- Entrez Gene: 16154 Mouse
- Entrez Gene: 117539 Rat
- Omim: 146933 Human
- SwissProt: Q13651 Human
- SwissProt: Q61727 Mouse
- Unigene: 504035 Human
- Unigene: 379327 Mouse
-
Alternative names
- CD210 antibody
- CDw210a antibody
- CDw210a antigen antibody
see all
Images
-
All lanes : Anti-IL-10RA antibody (ab225820) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) extract
Lane 3 : Mouse brain extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDaBlocking buffer: 3% non-fat dry milk in TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab225820 has been referenced in 5 publications.
- Zhu HS et al. Prior transfusion of umbilical cord mesenchymal stem cells can effectively alleviate symptoms of motion sickness in mice through interleukin 10 secretion. World J Stem Cells 13:177-192 (2021). PubMed: 33708346
- Peng Q et al. SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg Imbalance. Front Bioeng Biotechnol 9:737334 (2021). PubMed: 35087796
- Tang TT et al. Extracellular vesicle-encapsulated IL-10 as novel nanotherapeutics against ischemic AKI. Sci Adv 6:eaaz0748 (2020). PubMed: 32851154
- Coco G et al. Regulatory T cells promote corneal endothelial cell survival following transplantation via interleukin-10. Am J Transplant 20:389-398 (2020). PubMed: 31587452
- Dai WJ et al. Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision Pain. Evid Based Complement Alternat Med 2019:8413576 (2019). PubMed: 31885668