Overview

  • Product name

    Anti-IL-10RB/IL-10R2 antibody [3F9]
    See all IL-10RB/IL-10R2 primary antibodies
  • Description

    Rat monoclonal [3F9] to IL-10RB/IL-10R2
  • Host species

    Rat
  • Tested applications

    Suitable for: Flow Cyt, Functional Studiesmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human IL-10RB/IL-10R2.

  • General notes


    This antibody has been reported to function as a blocking agent.

    Previously labelled as IL10RB.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  • Primary antibody notes

    This antibody has been reported to function as a blocking agent.
  • Clonality

    Monoclonal
  • Clone number

    3F9
  • Myeloma

    Sp2/0
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/200.

Use 10µl of this dilution for 106 cells in 100µl. Due to low expression of CD210, sensitive staining techniques may be required.

 

 

 

 

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Functional Studies Use at an assay dependent concentration.

Target

  • Function

    Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IL29.
  • Involvement in disease

    Defects in IL10RB are the cause of inflammatory bowel disease type 25 (IBD25) [MIM:612567]. It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • Sequence similarities

    Belongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cd210 antibody
    • CDw210b antibody
    • CRF2 4 antibody
    • CRF2-4 antibody
    • CRFB4 antibody
    • Cytokine receptor class II antibody
    • Cytokine receptor class II CRF2 4 antibody
    • Cytokine receptor class-II member 4 antibody
    • Cytokine receptor family 2 member 4 antibody
    • Cytokine receptor family II member 4 antibody
    • D21S58 antibody
    • D21S66 antibody
    • I10R2_HUMAN antibody
    • IL-10 receptor subunit beta antibody
    • IL-10R subunit 2 antibody
    • IL-10R subunit beta antibody
    • IL-10R2 antibody
    • IL-10RB antibody
    • IL10R2 antibody
    • IL10RB antibody
    • Interleukin 10 receptor beta antibody
    • Interleukin-10 receptor subunit 2 antibody
    • Interleukin-10 receptor subunit beta antibody
    see all

References

ab33401 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



The concentrations noted on our online datasheets for these products are the only concentrations available at this time. However, one easy way to increase the concentration for these products would be to use a 10KdA moleuclar weight cut off spin column. Briefly, one just adds the antibody into the spin column and after a short centrifugation, buffer from the product flows through but protein is retained above the filter.

Here is a link to our 10kD spin column kit:
https://www.abcam.com/10kd-spin-column-ab93349.html

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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