Key features and details
- Rat monoclonal [AP-MAB0853] to IL-12
- Suitable for: WB, IP, Neutralising, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Product nameAnti-IL-12 antibody [AP-MAB0853]
See all IL-12 primary antibodies
DescriptionRat monoclonal [AP-MAB0853] to IL-12
Tested applicationsSuitable for: WB, IP, Neutralising, Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant fragment corresponding to Mouse IL-12.
Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).
ab80682 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Purification notesThe IgG fraction of culture supernatant was purified by Protein A affinity chromatography.
Primary antibody notesab80682 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
Our Abpromise guarantee covers the use of ab80682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 24 kDa.|
|IP||1/50 - 1/200.|
|Neutralising||Use at an assay dependent concentration. Neutralizes the bioactivity of natural or recombinant IL12 both in vivo and in vitro.|
|Flow Cyt||1/200 - 1/400.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Sequence similaritiesBelongs to the IL-6 superfamily.
- Information by UniProt
- CLMF antibody
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- CLMF p40 antibody
ab80682 has been referenced in 2 publications.
- Yamaguchi T et al. Proinflammatory M1 Macrophages Inhibit RANKL-Induced Osteoclastogenesis. Infect Immun 84:2802-12 (2016). PubMed: 27456834
- Zhou X et al. Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide. Mol Med Rep 12:7425-32 (2015). PubMed: 26459920