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During the chemical conjugation process, biotin is non-specifically attached to free groups on the antibody and multiple biotins will conjugate to a single antibody. For this reason, there is no single site at which biotin is attached to this antibody.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information
Heather AllenAbcam Scientific Support
Answered on Apr 15 2014