Overview

  • Product name

  • Description

    Goat polyclonal to IL-12 p40
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse IL-12 p40 aa 211-222 (internal sequence).
    Sequence:

    C-EARQQNKYENYS


    Database link: NP_032378.1

  • Positive control

    • Mouse Spleen and Rat Liver lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab77373 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/4000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
      Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
    • Involvement in disease

      Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
      Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 3 subfamily.
      Contains 1 fibronectin type-III domain.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • CLMF antibody
      • CLMF p40 antibody
      • CLMF2 antibody
      • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
      • IL 12 subunit p40 antibody
      • IL 12B antibody
      • IL-12 subunit p40 antibody
      • IL-12B antibody
      • IL12 p40 antibody
      • IL12 subunit p40 antibody
      • IL12B antibody
      • IL12B_HUMAN antibody
      • interleukin 12 beta chain antibody
      • Interleukin 12 p40 antibody
      • Interleukin 12 subunit beta antibody
      • Interleukin 12B antibody
      • Interleukin-12 subunit beta antibody
      • natural killer cell stimulatory factor 40 kD subunit antibody
      • NK cell stimulatory factor chain 2 antibody
      • NKSF antibody
      • NKSF2 antibody
      • p40 antibody
      see all

    Images

    • Anti-IL-12 p40 antibody (ab77373) at 1 µg/ml + Rat Liver lysate (in RIPA buffer) at 35 µg

      Predicted band size: 38 kDa
      Observed band size: 38 kDa



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    ab77373 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry. I can confirm we have the following IL-12 p40 antibodies available which have been tested in western blot.  I can recommend to review the online datasheets for further information, and to select one that has been tested and guaranteed in the species you are using: ab106270  Rabbit polyclonal Anti-IL12 p40 antibody Applications: ELISA, IHC-P, WB Reacts with: Human Click here (or use the following: https://www.abcam.com/index.html?datasheet=106270).         ab62822 Goat polyclonal Anti-IL12 p40 antibody Applications: ELISA, IHC-P, WB Reacts with: Human Click here (or use the following: https://www.abcam.com/index.html?datasheet=62822).         ab77373 Goat polyclonal Anti-IL12 p40 antibody Applications: ELISA, WB Reacts with: Mouse, Rat  Click here (or use the following: https://www.abcam.com/index.html?datasheet=77373). ab20196 Mouse monoclonal Anti-IL12 p40 antibody [1-1D5] Applications: ELISA, Neut, RIA, WB Reacts with: Human             Click here (or use the following: https://www.abcam.com/index.html?datasheet=20196). ab58940 Sheep polyclonal Anti-IL12 p40 antibody Applications: ICC/IF, WB Reacts with: Human Click here (or use the following: https://www.abcam.com/index.html?datasheet=58940). I am sorry to confirm that non of our IL-12 p40 antibodies have been tested in IP.  All tested and guaranteed applications (and species) are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. If you would like to test one of these antibodies in IP, please contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program. Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.  

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