Overview

  • Product name

    Anti-IL-12 p40 antibody [EPR20096-13]
    See all IL-12 p40 primary antibodies
  • Description

    Rabbit monoclonal [EPR20096-13] to IL-12 p40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    Anti-IL-12 p40 antibody (ab222632) is used in our 90-minute ELISA kit, Human IL-12 p40 ELISA kit (ab220656).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20096-13
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Target

  • Function

    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
    Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Involvement in disease

    Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLMF antibody
    • CLMF p40 antibody
    • CLMF2 antibody
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
    • IL 12 subunit p40 antibody
    • IL 12B antibody
    • IL-12 subunit p40 antibody
    • IL-12B antibody
    • IL12 p40 antibody
    • IL12 subunit p40 antibody
    • IL12B antibody
    • IL12B_HUMAN antibody
    • interleukin 12 beta chain antibody
    • Interleukin 12 p40 antibody
    • Interleukin 12 subunit beta antibody
    • Interleukin 12B antibody
    • Interleukin-12 subunit beta antibody
    • natural killer cell stimulatory factor 40 kD subunit antibody
    • NK cell stimulatory factor chain 2 antibody
    • NKSF antibody
    • NKSF2 antibody
    • p40 antibody
    see all

Images

  • Example of human IL-12 p40 standard curve in 1X Cell Extraction Buffer PTR using recombinant IL-12 p40 RabMAb antibody (ab222632) and a recombinant monoclonal antibody. Background-subtracted values (mean +/- SD) are graphed.

    For a complete ELISA kit, see Human IL-12 p40 ELISA kit (ab220656).

References

This product has been referenced in:

  • Hermes ML  et al. Gastrin-releasing peptide acts via postsynaptic BB2 receptors to modulate inward rectifier K+ and TRPV1-like conductances in rat paraventricular thalamic neurons. J Physiol 591:1823-39 (2013). Read more (PubMed: 23359674) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222632.
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