Overview

  • Product name
  • Description
    Rabbit polyclonal to IL-12 p70
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Neutralising, WBmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human IL-12 p70.

  • General notes
    Endotoxin level: 115 pg/ml (LAL test).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: PBS, 4.275% Trehalose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab25105 was sequentially purified by ammonium sulphate precipitation and protein A affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising
WB
  • Application notes
    Neut: Use 8000 neutralizing units/mg protein. One neutralizing unit is defined as the amount of IgG sufficient for a 50% inhibition of IFN gamma synthesis by human PBMC (2 x 105 cells) stimulated with 1 ng Human IL12.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 25 and 37 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      IL-12 p70 is a disulfide bonded heterodimer of the IL-12 40 kDa and 35 kDa subunits.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • IL12A antibody
      • IL12B antibody
      • CLMF p35 antibody
      • CLMF p40 antibody
      • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
      • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
      • Interleukin 12 alpha chain antibody
      • Interleukin-12 beta chain antibody
      • NK cell stimulatory factor chain 1 antibody
      • NK cell stimulatory factor chain 2 antibody
      • NKSF1 antibody
      • NKSF2 antibody
      see all

    References

    ab25105 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us again.

    Your customer is absolutely right. I am sorry about this, but I made a mistake by confusing two different cases, both were for rat samples. Please accept my apology.

    In this light, the homology is too low so that the antibody will probably not react with this species. As all of our IL12 p70 antibodies are raised against the recombinant full human protein, I am afraid none of our products is suitable.

    I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you very much for your interest in ab25105 Anti-IL12 p70 antibody.

    To our knowledge, ab25105 has not been tested in rat. However, the homology between the human protein and the rat protein is very high with 99-100%. Therefore, I can offer a discount off a future purchase if you buy ab25105 now, test it in ratand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    Ifthey are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that your customerwould like to proceed and test ab25105 in rat. We would need their email adress and the institution/companie they are working for.I will then send a discount code. This code must be issued before purchasing ab25105 so please wait for my reply before ordering.

    2. Purchase ab25105either by phone, fax, or online.

    3. Test it in rat.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab25105turns out to be unsuitable for rat,you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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