Anti-IL-12 p70 antibody [QS-12p70] (ab25036)
Key features and details
- Mouse monoclonal [QS-12p70] to IL-12 p70
- Suitable for: ELISA, ELISpot, Neutralising
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-IL-12 p70 antibody [QS-12p70] -
Description
Mouse monoclonal [QS-12p70] to IL-12 p70 -
Host species
Mouse -
Specificity
ab25036 recognises IL-12p70 heterodimer, but shows no reactivity with the p35 or p40 monomer. -
Tested applications
Suitable for: ELISA, ELISpot, Neutralisingmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IL-12 p70.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituents: PBS, 4.275% Trehalose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
QS-12p70 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | ||
ELISpot | ||
Neutralising |
Notes |
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ELISPOT: Use at an assay dependent dilution.
In vitro Neut: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
IL-12 p70 is a disulfide bonded heterodimer of the IL-12 40 kDa and 35 kDa subunits. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161560 Human
- Omim: 161561 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
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Alternative names
- IL12A antibody
- IL12B antibody
- CLMF p35 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab25036 has been referenced in 3 publications.
- Zheng G et al. Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab. Signal Transduct Target Ther 6:236 (2021). PubMed: 34158475
- Sierra JM et al. Tumor-Experienced Human NK Cells Express High Levels of PD-L1 and Inhibit CD8+ T Cell Proliferation. Front Immunol 12:745939 (2021). PubMed: 34616407
- Le Page ME et al. Killer Ig-like receptor 2DL4 does not mediate NK cell IFN-? responses to soluble HLA-G preparations. J Immunol 192:732-40 (2014). PubMed: 24337374