Overview

  • Product name

  • Description

    Goat polyclonal to IL-12A
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Goat, Horse, Cow, Cat, Dog, Pig, Baboon, Rhesus monkey, Deer, Llama
  • Immunogen

    Synthetic peptide corresponding to Human IL-12A aa 63-76 (internal sequence). NP_000873.2.
    Sequence:

    PCTSEEIDHEDITK


    Database link: P29459

  • Positive control

    • IHC-P: Human small intestine tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223235 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLMF antibody
    • CLMF p35 antibody
    • Cytotoxic lymphocyte maturation factor 1 p35 antibody
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
    • IL 12 subunit p35 antibody
    • IL 12A antibody
    • IL-12 subunit p35 antibody
    • IL-12A antibody
    • IL12A antibody
    • IL12A_HUMAN antibody
    • IL35 antibody
    • IL35 subunit antibody
    • Interleukin 12 alpha chain antibody
    • Interleukin 12 p35 antibody
    • Interleukin 12 subunit alpha antibody
    • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody
    • Interleukin-12 subunit alpha antibody
    • Natural killer cell stimulatory factor 1 35 kD subunit antibody
    • NF cell stimulatory factor chain 1 antibody
    • NFSK antibody
    • NK cell stimulatory factor chain 1 antibody
    • NKSF1 antibody
    • P35 antibody
    see all

Images

  • Paraffin-embedded human small intestine tissue stained for IL-12A using ab223235 at 3.8 μg/ml in immunohistochemical analysis, followed by AP-staining.

References

ab223235 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223235.
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