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Immunology Innate Immunity Cytokines Interleukins
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RecombinantRabMAb

Recombinant Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
  • Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
  • Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
  • Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP5737] to IL-12A - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-IL-12A antibody [EP5737] - BSA and Azide free
    See all IL-12A primary antibodies
  • Description

    Rabbit monoclonal [EP5737] to IL-12A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human IL-12A aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • NIH3T3, HuT-78, and Raw 264.7 cell lysates; Human kidney tissue, Human gastric carcinoma
  • General notes

    ab214433 is the carrier-free version of ab131039 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab214433 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP5737
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins

Associated products

  • Alternative Versions

    • Anti-IL-12A antibody [EP5737] (ab131039)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH 3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant Human IL-12A protein (Fc Chimera) (ab221208)

Applications

Our Abpromise guarantee covers the use of ab214433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P29459 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3592 Human
    • Entrez Gene: 16159 Mouse
    • Omim: 161560 Human
    • SwissProt: P29459 Human
    • SwissProt: P43431 Mouse
    • Unigene: 673 Human
    • Unigene: 103783 Mouse
    • Alternative names

      • CLMF antibody
      • CLMF p35 antibody
      • Cytotoxic lymphocyte maturation factor 1 p35 antibody
      • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
      • IL 12 subunit p35 antibody
      • IL 12A antibody
      • IL-12 subunit p35 antibody
      • IL-12A antibody
      • IL12A antibody
      • IL12A_HUMAN antibody
      • IL35 antibody
      • IL35 subunit antibody
      • Interleukin 12 alpha chain antibody
      • Interleukin 12 p35 antibody
      • Interleukin 12 subunit alpha antibody
      • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody
      • Interleukin-12 subunit alpha antibody
      • Natural killer cell stimulatory factor 1 35 kD subunit antibody
      • NF cell stimulatory factor chain 1 antibody
      • NFSK antibody
      • NK cell stimulatory factor chain 1 antibody
      • NKSF1 antibody
      • P35 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

      Immunohistochemical staining of paraffin embedded human gastric carcinoma tissue section labelling IL12A with purified ab131039 at dilution of 1/800. The secondary antibody used was HRP conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

    • Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

      Flow cytometry analysis of THP-1 cells treated with 100ng/ml PMA for 30min,then 1µg/ml LPS for 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Dylight488® (Ab98462) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue). Untreated cells (Green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

    • Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

      Flow cytometry analysis of Raw264.7 cells treated with 100ng/ml for LPS 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labelling IL12A with unpurified ab131039 at dilution of 1:250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)This image is courtesy of an anonymous Abreview.

      Formaldehyde-fixed, paraffin-embedded human colon tissue stained for IL12A using ab131039 at 1/500 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab214433? Please let us know so that we can cite the reference in this datasheet.

    ab214433 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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