Recombinant
RabMAb

Recombinant Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

Submit a review Submit a question

Overview

  • Product name

    Anti-IL-12A antibody [EP5737] - BSA and Azide free
    See all IL-12A primary antibodies

  • Description

    Rabbit monoclonal [EP5737] to IL-12A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human IL-12A aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • NIH3T3, HuT-78, and Raw 264.7 cell lysates; Human kidney tissue, Human gastric carcinoma

  • General notes

    ab214433 is the carrier-free version of ab131039 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab214433 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab214433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

  • Sequence similarities

    Belongs to the IL-6 superfamily.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • CLMF antibody
      • CLMF p35 antibody
      • Cytotoxic lymphocyte maturation factor 1 p35 antibody
      • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
      • IL 12 subunit p35 antibody
      • IL 12A antibody
      • IL-12 subunit p35 antibody
      • IL-12A antibody
      • IL12A antibody
      • IL12A_HUMAN antibody
      • IL35 antibody
      • IL35 subunit antibody
      • Interleukin 12 alpha chain antibody
      • Interleukin 12 p35 antibody
      • Interleukin 12 subunit alpha antibody
      • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody
      • Interleukin-12 subunit alpha antibody
      • Natural killer cell stimulatory factor 1 35 kD subunit antibody
      • NF cell stimulatory factor chain 1 antibody
      • NFSK antibody
      • NK cell stimulatory factor chain 1 antibody
      • NKSF1 antibody
      • P35 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

      Immunohistochemical staining of paraffin embedded human gastric carcinoma tissue section labelling IL12A with purified ab131039 at dilution of 1/800. The secondary antibody used was HRP conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

    • Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

      Flow cytometry analysis of THP-1 cells treated with 100ng/ml PMA for 30min,then 1µg/ml LPS for 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Dylight488® (Ab98462) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue). Untreated cells (Green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

    • Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Flow Cytometry - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

      Flow cytometry analysis of Raw264.7 cells treated with 100ng/ml for LPS 24hour cells labelling IL-12A with ab131039 at 1/19000 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labelling IL12A with unpurified ab131039 at dilution of 1:250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12A antibody [EP5737] - BSA and Azide free (ab214433)This image is courtesy of an anonymous Abreview.

      Formaldehyde-fixed, paraffin-embedded human colon tissue stained for IL12A using ab131039 at 1/500 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131039).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab214433 has not yet been referenced specifically in any publications.

    Publishing research using ab214433? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab214433.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com