Product nameAnti-IL-12A antibody [EPR5736]
See all IL-12A primary antibodies
DescriptionRabbit monoclonal [EPR5736] to IL-12A
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human IL-12A aa 50-150.
Database link: P29459
- Human IL-12A recombinant protein
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.50 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 25 kDa).|
|IP||1/10 - 1/100.|
FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Sequence similaritiesBelongs to the IL-6 superfamily.
- Information by UniProt
- CLMF antibody
- CLMF p35 antibody
- Cytotoxic lymphocyte maturation factor 1 p35 antibody
Anti-IL-12A antibody [EPR5736] (ab133751) at 1/1000 dilution + Human IL-12A recombinant protein at 0.01 µg
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Human IL-12A recombinant protein has a predicted MWt of 29 kDa.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Liu S et al. Silica Particles Mediate Phenotypic and Functional Alteration of Dendritic Cells and Induce Th2 Cell Polarization. Front Immunol 10:787 (2019). Read more (PubMed: 31068929) »
- Reitberger S et al. Assembly-induced folding regulates interleukin 12 biogenesis and secretion. J Biol Chem 292:8073-8081 (2017). Read more (PubMed: 28325840) »