Overview

  • Product name

    Anti-IL-12A antibody [EPR5736]
    See all IL-12A primary antibodies
  • Description

    Rabbit monoclonal [EPR5736] to IL-12A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human IL-12A aa 50-150.
    Database link: P29459

  • Positive control

    • Human IL-12A recombinant protein
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 25 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
    • Sequence similarities

      Belongs to the IL-6 superfamily.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • CLMF antibody
      • CLMF p35 antibody
      • Cytotoxic lymphocyte maturation factor 1 p35 antibody
      • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
      • IL 12 subunit p35 antibody
      • IL 12A antibody
      • IL-12 subunit p35 antibody
      • IL-12A antibody
      • IL12A antibody
      • IL12A_HUMAN antibody
      • IL35 antibody
      • IL35 subunit antibody
      • Interleukin 12 alpha chain antibody
      • Interleukin 12 p35 antibody
      • Interleukin 12 subunit alpha antibody
      • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody
      • Interleukin-12 subunit alpha antibody
      • Natural killer cell stimulatory factor 1 35 kD subunit antibody
      • NF cell stimulatory factor chain 1 antibody
      • NFSK antibody
      • NK cell stimulatory factor chain 1 antibody
      • NKSF1 antibody
      • P35 antibody
      see all

    Images

    • Anti-IL-12A antibody [EPR5736] (ab133751) at 1/1000 dilution + Human IL-12A recombinant protein at 0.01 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa
      Observed band size: 29 kDa
      why is the actual band size different from the predicted?



      Human IL-12A recombinant protein has a predicted MWt of 29 kDa.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Liu S  et al. Silica Particles Mediate Phenotypic and Functional Alteration of Dendritic Cells and Induce Th2 Cell Polarization. Front Immunol 10:787 (2019). Read more (PubMed: 31068929) »
    • Reitberger S  et al. Assembly-induced folding regulates interleukin 12 biogenesis and secretion. J Biol Chem 292:8073-8081 (2017). Read more (PubMed: 28325840) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133751.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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