Recombinant Anti-IL-12B antibody [EPR5739] (ab133752)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5739] to IL-12B
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-12B antibody [EPR5739]
See all IL-12B primary antibodies -
Description
Rabbit monoclonal [EPR5739] to IL-12B -
Host species
Rabbit -
Specificity
This antibody is unsuitable to detect endogenous IL-12B in WB.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human IL-12B recombinant protein. ICC/IF: MOLT-4 cells Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.30 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5739 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/300.
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WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
This antibody is unsuitable to detect endogenous IL12B in WB |
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/300. |
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). This antibody is unsuitable to detect endogenous IL12B in WB |
ICC/IF
1/100 - 1/250. |
Target
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
Involvement in disease
Mendelian susceptibility to mycobacterial disease
Psoriasis 11 -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Post-translational
modificationsKnown to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- SwissProt: P29460 Human
- Unigene: 674 Human
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Alternative names
- CLMF antibody
- CLMF p40 antibody
- CLMF2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling IL-12B with Purified ab133752 at 1:100 dilution (3.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were not counterstained. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution (Purified)
All lanes : Human IL-12 recombinant protein
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?The recombinant protein contains Human IL-12B (Ile23-Ser328) and Human IL-12A (Arg23-Ser219) connected by disulfide bond to be heterodimer. Under reducing conditions, the observed bands will be at 40 KD for IL-12B and 29 KD for IL-12A.
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Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution + Human IL-12B recombinant protein at 0.01 µg
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This image was generated using the unpurified version of the product.
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Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling IL-12B (red) with ab133752 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab133752 has been referenced in 5 publications.
- Mideksa YG et al. A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines. J Biol Chem 298:102677 (2022). PubMed: 36336075
- Bohnacker S et al. Influence of glycosylation on IL-12 family cytokine biogenesis and function. Mol Immunol 126:120-128 (2020). PubMed: 32823236
- Meier S et al. The molecular basis of chaperone-mediated interleukin 23 assembly control. Nat Commun 10:4121 (2019). PubMed: 31511508
- Reitberger S et al. Assembly-induced folding regulates interleukin 12 biogenesis and secretion. J Biol Chem 292:8073-8081 (2017). PubMed: 28325840
- Marks E et al. Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation. Mucosal Immunol 10:1224-1236 (2017). PubMed: 28120851