Key features and details
- Rabbit polyclonal to IL-12RB2 - C-terminal
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-12RB2 antibody - C-terminal
See all IL-12RB2 primary antibodies
DescriptionRabbit polyclonal to IL-12RB2 - C-terminal
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human IL-12RB2 aa 756-783 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q99665
- IHC-P: Human liver tissue. WB: MDA-MB435 cell lysate. Flow Cyt: MDA-MB435 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 97 kDa.|
|Flow Cyt||1/10 - 1/50.|
FunctionReceptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
Tissue specificityIsoform 2 is expressed at similar levels in both naive and activated T-cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Developmental stageMaximum levels in Th1 cells between day 3 and day 8 of activation.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
modificationsOn IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding.
- Information by UniProt
- I12R2_HUMAN antibody
- IL-12 receptor subunit beta-2 antibody
- IL-12R subunit beta-2 antibody
Flow cytometric analysis of MDA-MB435 cell line labeling IL-12RB2 with ab230446 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for IL-12RB2 using ab230446 at 1/50 dilution in immunohistochemical analysis.
Anti-IL-12RB2 antibody - C-terminal (ab230446) at 1/1000 dilution + MDA-MB435 cell lysate at 35 µg
Predicted band size: 97 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230446 has not yet been referenced specifically in any publications.