Overview

  • Product name

    Anti-IL-12RB2 antibody - C-terminal
    See all IL-12RB2 primary antibodies
  • Description

    Rabbit polyclonal to IL-12RB2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IL-12RB2 aa 756-783 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q99665

  • Positive control

    • IHC-P: Human liver tissue. WB: MDA-MB435 cell lysate. Flow Cyt: MDA-MB435 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 97 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
  • Tissue specificity

    Isoform 2 is expressed at similar levels in both naive and activated T-cells.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
  • Developmental stage

    Maximum levels in Th1 cells between day 3 and day 8 of activation.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications

    On IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • I12R2_HUMAN antibody
    • IL-12 receptor subunit beta-2 antibody
    • IL-12R subunit beta-2 antibody
    • IL-12R-beta-2 antibody
    • IL-12RB2 antibody
    • IL12 receptor beta 2 antibody
    • IL12R beta2 antibody
    • IL12RB2 antibody
    • Interleukin 12 receptor beta 2 antibody
    • Interleukin 12 receptor beta 2 chain antibody
    • Interleukin-12 receptor subunit beta-2 antibody
    • RP11 102M16.1 antibody
    see all

Images

  • Flow cytometric analysis of MDA-MB435 cell line labeling IL-12RB2 with ab230446 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for IL-12RB2 using ab230446 at 1/50 dilution in immunohistochemical analysis.

  • Anti-IL-12RB2 antibody - C-terminal (ab230446) at 1/1000 dilution + MDA-MB435 cell lysate at 35 µg

    Predicted band size: 97 kDa

References

ab230446 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab230446.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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