Key features and details
- Rat monoclonal [13A] to IL-13 (PE)
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product nameAnti-IL-13 antibody [13A] (PE)
See all IL-13 primary antibodies
DescriptionRat monoclonal [13A] to IL-13 (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse IL-13.
- BALB/c splenocytes
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab95576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.25µg for 105-8 cells.
ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
Involvement in diseaseAllergic rhinitis
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- Allergic rhinitis antibody
- ALRH antibody
- BHR 1 antibody
ab95576 at 0.125 µg/test staining IL-13 by Flow Cytometry.
BALB/c splenocytes were cultured for 3 days with plate-bound Anti-Mouse CD3e, soluble Anti-Mouse CD28, Mouse IL2 Recombinant Protein and Mouse IL4 Recombinant Protein. Cells were then harvested and restimulated with PMA and Ionomycin in the presence of monensin for 5 hours. Following restimulation, cells were harvested, surface stained with Anti-Mouse CD4 FITC, fixed and permeabilized, then stained with 0.125 µg of ab95576 (1). The ligand blocking control demonstrates that pre-blocking ab95576 with 1.0 µg of Mouse IL-13 Recombinant Protein prior to staining abrogates specific mouse IL-13 staining (2).
ab95576 has been referenced in 1 publication.
- Poe C et al. Treatment of established TH2 cells with 4µ8c, an inhibitor of IRE1a, blocks IL-5 but not IL-4 secretion. BMC Immunol 20:3 (2019). PubMed: 30630412