Overview

  • Product name

    Anti-IL-13 antibody [13A] (Phycoerythrin)
    See all IL-13 primary antibodies
  • Description

    Rat monoclonal [13A] to IL-13 (Phycoerythrin)
  • Host species

    Rat
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse IL-13.

  • Positive control

    • BALB/c splenocytes

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25µg for 105-8 cells.

ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease

    Allergic rhinitis
  • Sequence similarities

    Belongs to the IL-4/IL-13 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR 1 antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • interleukin13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC 30 antibody
    • NC30 antibody
    • P 600 antibody
    • P600 antibody
    see all

Images

  • ab95576 at 0.125 µg/test staining IL-13 by Flow Cytometry.
    BALB/c splenocytes were cultured for 3 days with plate-bound Anti-Mouse CD3e, soluble Anti-Mouse CD28, Mouse IL2 Recombinant Protein and Mouse IL4 Recombinant Protein. Cells were then harvested and restimulated with PMA and Ionomycin in the presence of monensin for 5 hours. Following restimulation, cells were harvested, surface stained with Anti-Mouse CD4 FITC, fixed and permeabilized, then stained with 0.125 µg of ab95576 (1). The ligand blocking control demonstrates that pre-blocking ab95576 with 1.0 µg of Mouse IL-13 Recombinant Protein prior to staining abrogates specific mouse IL-13 staining (2).

References

This product has been referenced in:

  • Poe C  et al. Treatment of established TH2 cells with 4µ8c, an inhibitor of IRE1a, blocks IL-5 but not IL-4 secretion. BMC Immunol 20:3 (2019). Read more (PubMed: 30630412) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab95576.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up