Anti-IL-13 antibody [A130D 12G5 1E4] (ab52538)
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- References
- Protocols
Overview
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Product name
Anti-IL-13 antibody [A130D 12G5 1E4]
See all IL-13 primary antibodies -
Description
Mouse monoclonal [A130D 12G5 1E4] to IL-13 -
Host species
Mouse -
Specificity
Recognizes recombinant and native rat IL-13 -
Tested applications
Suitable for: WB, ELISA, Flow Cyt, Dot blotmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant full length protein corresponding to Rat IL-13.
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Positive control
- FACS: intracellular IL-13 can be detected in PMA/ionophore-stimulated rat splenocytes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
>95% pure as determined by SDS-PAGE analysis. 0.22 micron sterile filtered. -
Clonality
Monoclonal -
Clone number
A130D 12G5 1E4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab52538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 14 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| Flow Cyt | Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Dot blot | 1/500 - 1/1000. |
Target
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Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 116553 Rat
- SwissProt: P42203 Rat
- Unigene: 9921 Rat
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Alternative names
- Allergic rhinitis antibody
- ALRH antibody
- BHR 1 antibody
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Datasheets and documents
References
ab52538 has not yet been referenced specifically in any publications.
