Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Indirect ELISA, Neutralising, Sandwich ELISA, WBmore details
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse IL-13.

Properties

Applications

Our Abpromise guarantee covers the use of ab109815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml.

(using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL-13.

Neutralising Use a concentration of 0.08 - 0.1 µg/ml.

to yield one-half maximal inhibition [ND50] of the biological activity of IL-13 (10.0 ng/ml)

Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL-13 (Biotin) (ab83162).

Using ab83162 as the detection antibody and ab109815 as the capture antibody, the detection limit for recombinant Mouse IL-13 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa.

The detection limit for recombinant Mouse IL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function

    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease

    Allergic rhinitis
  • Sequence similarities

    Belongs to the IL-4/IL-13 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR 1 antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • interleukin13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC 30 antibody
    • NC30 antibody
    • P 600 antibody
    • P600 antibody
    see all

References

This product has been referenced in:

  • McSorley HJ  et al. Expansion of Foxp3+ regulatory T cells in mice infected with the filarial parasite Brugia malayi. J Immunol 181:6456-66 (2008). Read more (PubMed: 18941236) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Raw 264.7)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
5 µg
Treatment
25 ng/ml LPS for 6, 12, 24, 36 and 48h
Specification
Raw 264.7
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 19 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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