• Product name

  • Description

    Rabbit polyclonal to IL-13
  • Host species

  • Tested applications

    Suitable for: WB, Neutralising, IHC-P, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)

  • Positive control

    • Recombinant human IL-13 protein (ab9577) can be used as a positive control in WB. IHC-P: human testis tissue sections



Our Abpromise guarantee covers the use of ab9576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
Neutralising Use a concentration of 0.12 - 0.13 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/ml).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 µg/ml.

Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution).


  • Function

    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease

    Allergic rhinitis
  • Sequence similarities

    Belongs to the IL-4/IL-13 family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR 1 antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • interleukin13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC 30 antibody
    • NC30 antibody
    • P 600 antibody
    • P600 antibody
    see all


  • All lanes : Anti-IL-13 antibody (ab9576) at 1/3000 dilution

    Lane 1 : E. coli expressed human recombinant protein IL-13 from supplier 1 at 0.2 µg
    Lane 2 : E. coli expressed human recombinant protein IL-13 from supplier 1 at 0.3 µg
    Lane 3 : E. coli expressed human recombinant protein IL-13 from supplier 2 at 0.2 µg
    Lane 4 : E. coli expressed human recombinant protein IL-13 from supplier 2 at 0.3 µg
    Lane 5 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.2 µg
    Lane 6 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.3 µg
    Lane 7 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.4 µg

    All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Observed band size: 10 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 26 kDa (possible dimer)

    Exposure time: 5 minutes

    15% denaturing gel.  Blot was blocked with 2% milk for 16 hours at 4°C and incubated with the primary antibody for 1 hour at 25°C.

    See Abreview

  • IHC image of IL-13 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9576, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Calibration curve of a sandwich assay for IL-13 using ab9576 as a detection antibody at a concentration of 0.5μg/ml.


This product has been referenced in:

  • Kadin ME  et al. IL-13 is produced by tumor cells in Breast Implant Associated Anaplastic Large Cell Lymphoma: implications for pathogenesis. Hum Pathol N/A:N/A (2018). Read more (PubMed: 29689246) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Lung)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C

Abcam user community

Verified customer

Submitted Aug 01 2016

Western blot
Human Recombinant protein (E.coli expressed commercial protein)
Gel Running Conditions
Non-reduced Denaturing (15%)
Loading amount
0.3 µg
E.coli expressed commercial protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Miss. Fenny Chong

Verified customer

Submitted Mar 11 2008

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