Key features and details
- Rabbit polyclonal to IL-13
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: unknown
Product nameAnti-IL-13 antibody
See all IL-13 primary antibodies
DescriptionRabbit polyclonal to IL-13
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)
- WB: Recombinant human IL-13 protein (ab9577).
Although some customers have been successful in IHC we no longer batch test in this application.
FormLyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml.
Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution).
FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
Involvement in diseaseAllergic rhinitis
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- Allergic rhinitis antibody
- ALRH antibody
- BHR 1 antibody
All lanes : Anti-IL-13 antibody (ab9576) at 1/3000 dilution
Lane 1 : E. coli expressed human recombinant protein IL-13 from supplier 1 at 0.2 µg
Lane 2 : E. coli expressed human recombinant protein IL-13 from supplier 1 at 0.3 µg
Lane 3 : E. coli expressed human recombinant protein IL-13 from supplier 2 at 0.2 µg
Lane 4 : E. coli expressed human recombinant protein IL-13 from supplier 2 at 0.3 µg
Lane 5 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.2 µg
Lane 6 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.3 µg
Lane 7 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.4 µg
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Observed band size: 10 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible dimer)
Exposure time: 5 minutes
15% denaturing gel. Blot was blocked with 2% milk for 16 hours at 4°C and incubated with the primary antibody for 1 hour at 25°C.
Calibration curve of a sandwich assay for IL-13 using ab9576 as a detection antibody at a concentration of 0.5μg/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9576 has been referenced in 2 publications.
- Kadin ME et al. IL-13 is produced by tumor cells in Breast Implant Associated Anaplastic Large Cell Lymphoma: implications for pathogenesis. Hum Pathol N/A:N/A (2018). PubMed: 29689246
- Oue S et al. Increased IL-13 expression is independently associated with neo-osteogenesis in patients with chronic rhinosinusitis. J Allergy Clin Immunol 140:1444-1448.e11 (2017). PubMed: 28601687