Product nameAnti-IL-13 antibody
See all IL-13 primary antibodies
DescriptionRabbit polyclonal to IL-13
Tested applicationsSuitable for: WB, Neutralising, IHC-P, Sandwich ELISAmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)
- Recombinant human IL-13 protein (ab9577) can be used as a positive control in WB. IHC-P: human testis tissue sections
FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPBS, pH 7.4, no preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Neutralising||Use a concentration of 0.12 - 0.13 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/ml).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml.
Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution).
FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
Involvement in diseaseAllergic rhinitis
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- Allergic rhinitis antibody
- ALRH antibody
- BHR1 antibody
All lanes : Anti-IL-13 antibody (ab9576) at 1/3000 dilution
Lane 1 : E. coli expressed human recombinant protein IL-13 from supplier 1 at 0.2 µg
Lane 2 : E. coli expressed human recombinant protein IL-13 from supplier 1 at 0.3 µg
Lane 3 : E. coli expressed human recombinant protein IL-13 from supplier 2 at 0.2 µg
Lane 4 : E. coli expressed human recombinant protein IL-13 from supplier 2 at 0.3 µg
Lane 5 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.2 µg
Lane 6 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.3 µg
Lane 7 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.4 µg
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Observed band size: 10 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible dimer)
Exposure time: 5 minutes
15% denaturing gel. Blot was blocked with 2% milk for 16 hours at 4°C and incubated with the primary antibody for 1 hour at 25°C.
IHC image of IL-13 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9576, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Calibration curve of a sandwich assay for IL-13 using ab9576 as a detection antibody at a concentration of 0.5μg/ml.