Recombinant HRP Anti-IL-13 antibody [EP3266] (ab201470)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP3266] to IL-13
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-IL-13 antibody [EP3266]
See all IL-13 primary antibodies -
Description
HRP Rabbit monoclonal [EP3266] to IL-13 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human IL-13 recombinant protein.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3266 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). |
Target
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Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3596 Human
- Entrez Gene: 16163 Mouse
- Entrez Gene: 116553 Rat
- Omim: 147683 Human
- SwissProt: P35225 Human
- SwissProt: P20109 Mouse
- SwissProt: P42203 Rat
- Unigene: 845 Human
see all -
Alternative names
- Allergic rhinitis antibody
- ALRH antibody
- BHR 1 antibody
see all
Images
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HRP Anti-IL-13 antibody [EP3266] (ab201470) at 1/5000 dilution + Human IL-13 Recombinant Protein at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201470 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab201470 has been referenced in 1 publication.
- Rafikov R et al. Cytokine profiling in pulmonary arterial hypertension: the role of redox homeostasis and sex. Transl Res 247:1-18 (2022). PubMed: 35405322