Overview

  • Product name
    Anti-IL-13 receptor alpha 1 antibody
    See all IL-13 receptor alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to IL-13 receptor alpha 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human IL-13 receptor alpha 1 (phospho Y405). (D-I-YP-E-K)

  • Positive control
    • NIH3T3 cells; COLO, HT29, A549 and 293 cell extracts; human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/40000.

Target

  • Function
    Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
  • Tissue specificity
    Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 5 subfamily.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • bB128O4.2.1 (interleukin 13 receptor, alpha 1) antibody
    • Cancer/testis antigen 19 antibody
    • CD213a1 antibody
    • CD213A1 Antigen antibody
    • CT 19 antibody
    • CT19 antibody
    • I13R1_HUMAN antibody
    • IL 13 receptor subunit alpha 1 antibody
    • IL 13R alpha 1 antibody
    • IL 13R subunit alpha 1 antibody
    • IL 13Ra antibody
    • IL 13RA1 antibody
    • IL-13 receptor subunit alpha-1 antibody
    • IL-13R subunit alpha-1 antibody
    • IL-13R-alpha-1 antibody
    • IL-13RA1 antibody
    • IL13 receptor alpha 1 chain antibody
    • IL13R antibody
    • IL13RA antibody
    • Il13ra1 antibody
    • Interleukin 13 receptor alpha 1 antibody
    • Interleukin 13 receptor alpha 1 chain antibody
    • interleukin 13 receptor subunit alpha 1 antibody
    • Interleukin-13 receptor subunit alpha-1 antibody
    • NR 4 antibody
    • NR4 antibody
    see all

Images

  • ab79277 at 1/50 dilution staining IL-13 receptor alpha 1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising peptide.

  • ab79277 at 1/500 dilution staining IL-13 receptor alpha 1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

  • All lanes : Anti-IL-13 receptor alpha 1 antibody (ab79277) at 1/500 dilution

    Lane 1 : COLO cell extracts
    Lane 2 : HT29 cell extracts
    Lane 3 : A549 cell extracts
    Lane 4 : 293 cell extracts
    Lane 5 : COLO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rhesus monkey Cell (Colon)
Specification
Colon
Blocking step
3% BSA and 10% Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 21 2013

Answer

Thank you for confirming these details and for your cooperation. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with one unit of anti-IL13 receptor alpha 1 ab79277. The order number is ********. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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