Product nameAnti-IL-13 receptor alpha 2 antibody [EPR22978-163]
See all IL-13 receptor alpha 2 primary antibodies
DescriptionRabbit monoclonal [EPR22978-163] to IL-13 receptor alpha 2
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human IL-13 receptor alpha 2 aa 1-350. The exact sequence is proprietary.
Database link: Q14627
- WB: Human testis, A375, A-172 and Daudi lysates. ICC/IF: A375 cells. IP: A375 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab260044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 44 kDa.
FunctionBinds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- Cancer/testis antigen 19 antibody
- CD213a2 antibody
- CT19 antibody
All lanes : Anti-IL-13 receptor alpha 2 antibody [EPR22978-163] (ab260044) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : A375 (human malignant melanoma epithelial cell) whole cell lysate
Lane 3 : A-172 (human brain glioblastoma) whole cell lysate
Lane 4 : Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution
Predicted band size: 44 kDa
Observed band size: 55,65 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 30718742).
Negative control: Daudi (PMID: 30718742).
Exposure time: Lane 1: 3 minutes; Lane 2: 10 seconds; Lanes 3-4: 3 minutes.
IL-13 receptor alpha 2 was immunoprecipitated from 0.35 mg A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg with ab260044 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260044 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg
Lane 2: ab260044 IP in A375 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260044 in A375 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A375 (human malignant melanoma epithelial cell) cells labeling IL-13 receptor alpha 2 with ab260044 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in A375 cell line.
Negative control: Daudi (PMID: 26059190.
ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab260044 has not yet been referenced specifically in any publications.