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    il-15-antibody-ab166923.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-15 antibody (ab166923)

  • Datasheet
  • SDS
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Western blot - Anti-IL-15 antibody (ab166923)

    Key features and details

    • Goat polyclonal to IL-15
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-IL-15 antibody [EPR22635-214] (ab273625)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-IL-15 antibody
      See all IL-15 primary antibodies
    • Description

      Goat polyclonal to IL-15
    • Host species

      Goat
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey
    • Immunogen

      Synthetic peptide corresponding to Human IL-15 aa 70-82 (internal sequence) (Cysteine residue). (NP_000576.1; NP_751915.1)
      Sequence:

      DATLYTESDVHPS

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Human Bone Marrow lysates.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 99% Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab166923 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • NK Cells
      • Metabolism
      • Types of disease
      • Obesity

    Associated products

    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant human IL-15 protein (Active) (ab259403)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab166923 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 18 kDa.

    1 hour primary incubation is recommended for this product.

    Notes
    WB
    Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 18 kDa.

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
    • Tissue specificity

      Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.
    • Sequence similarities

      Belongs to the IL-15/IL-21 family.
    • Cellular localization

      Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
    • Target information above from: UniProt accession P40933 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 281248 Cow
      • Entrez Gene: 100034058 Horse
      • Entrez Gene: 3600 Human
      • Entrez Gene: 397683 Pig
      • Entrez Gene: 100009017 Rabbit
      • Omim: 600554 Human
      • SwissProt: Q28028 Cow
      • SwissProt: P40933 Human
      • SwissProt: Q95253 Pig
      • SwissProt: Q3Y5G8 Rabbit
      • Unigene: 654378 Human
      see all
    • Alternative names

      • IL 15 antibody
      • IL-15 antibody
      • IL15 antibody
      • IL15_HUMAN antibody
      • Interleukin 15 antibody
      • Interleukin-15 antibody
      • Interleukin15 antibody
      • MGC9721 antibody
      see all

    Images

    • Western blot - Anti-IL-15 antibody (ab166923)
      Western blot - Anti-IL-15 antibody (ab166923)
      Anti-IL-15 antibody (ab166923) at 1 µg/ml + Human Bone Marrow lysate at 35 µg

      Developed using the ECL technique.

      Predicted band size: 18 kDa



      Primary incubation was 1 hour

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab166923? Please let us know so that we can cite the reference in this datasheet.

    ab166923 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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