Overview

  • Product name
  • Description
    Rabbit polyclonal to IL-15
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Blocking, ICC/IF, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse IL-15.

  • General notes


    IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use a concentration of 10 µg/ml. PubMed: 17239632
ICC/IF Use a concentration of 1 µg/ml. PubMed: 15921960
Neutralising Use at an assay dependent concentration.
WB 1/500.

Target

  • Function
    Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
  • Tissue specificity
    Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.
  • Sequence similarities
    Belongs to the IL-15/IL-21 family.
  • Cellular localization
    Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 15 antibody
    • IL-15 antibody
    • IL15 antibody
    • IL15_HUMAN antibody
    • Interleukin 15 antibody
    • Interleukin-15 antibody
    • Interleukin15 antibody
    • MGC9721 antibody
    see all

References

This product has been referenced in:
  • Du YE  et al. MiR-205/YAP1 in Activated Fibroblasts of Breast Tumor Promotes VEGF-independent Angiogenesis through STAT3 Signaling. Theranostics 7:3972-3988 (2017). Read more (PubMed: 29109792) »
  • Liu Q  et al. Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation. Nat Neurosci 19:243-52 (2016). Read more (PubMed: 26752157) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Small intestine)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: alpha chemotrypsin
Specification
Small intestine
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Jun 02 2017

Answer

DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab7213 in Flow Cytometry on Mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry on Mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for your enquiry. All the information that we have regarding ab7213 is located on the online datasheet. I'm sorry, but it does not appear that we have information about a positive control for this particular antibody. If you have any more questions, please contact us again.

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Answer

Sorry, we do not have a biotinylated form of this antibody, and we do not have a service to perfom this.

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Answer

This antibody has been shown to detect mouse IL-15 in WB. The only references we are aware of are those listed on the datasheet.

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Answer

The immunogen used to create this antibody was Mouse IL-15. The antibody is a polyclonal and will therefore recognise a number of epitopes on the surface of the immunogen. As far as we are aware, the antibody has not been tested in flow cytometry.

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Answer

This antibody is neutralizing, but you will have to establish your own conditions.

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Answer

This antibody has not been tested for neutralization.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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