Overview

  • Product name
    Anti-IL-15 antibody [EP433Y]
    See all IL-15 primary antibodies
  • Description
    Rabbit monoclonal [EP433Y] to IL-15
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for IL-15.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant full length protein within Human IL-15. The exact sequence is proprietary.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab40668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 31 kDa (predicted molecular weight: 18 kDa).

Target

  • Function
    Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
  • Tissue specificity
    Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.
  • Sequence similarities
    Belongs to the IL-15/IL-21 family.
  • Cellular localization
    Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
  • Information by UniProt
  • Database links
  • Alternative names
    • IL 15 antibody
    • IL-15 antibody
    • IL15 antibody
    • IL15_HUMAN antibody
    • Interleukin 15 antibody
    • Interleukin-15 antibody
    • Interleukin15 antibody
    • MGC9721 antibody
    see all

Images

References

ab40668 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.

As discussed over the phone, I have been looking into how the anti-IL15 antibodies ab40668 and ab134177 have been tested and whether they would be the most suitable antibodies to use in your experiments.

As discussed over the phone, the bands observed with ab40668 and ab134177 in Western blotting are not exactly where they would be expected to run as recombinant fusion proteins were used. With ab40668 as fusion protein of IL-15 (aa49-162, 12kDa) and Thioredoxin-1 (aa1-109, 12kDa), plus His tag (7kDa) was used, bringing the expected molecular weight to ˜ 31 kDa which is what is observed on the blot. The antibody ab134177 used a slightly truncated version of the IL15, making the expected band size ˜12 kDa (as observed).

Unfortunately, both of these antibodies have been tried with samples containing endogenously expressed IL15 and we have been unable to detect a signal. It may be that the conditions necessary for using these antibodies with endogenous samples requires some further optimisation. I would suggest, as you are intending to use the antibodies with endogenous samples to have a look at the ab7213 or ab109082 instead. Both of these would be covered by the Abpromise guarantee for the application and species you intend to use the antibody for.

More information on the Abpromise can be found from the following link:

www.abcam.com/abpromise

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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