Product nameIL-15 overexpression 293T lysate (whole cell)
DescriptionIL-15 overexpression lysate
ab94112 is a 293T cell transfected lysate in which Human IL-15 has been transiently over-expressed using a pCMV-IL-15 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. Tissue specificity: Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus. Similarity: Belongs to the IL-15/IL-21 family.
Our Abpromise guarantee covers the use of ab94112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94112 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-IL-15 antibody (ab55276) at 1/500 dilution
Lane 1 :
IL-15 overexpression 293T lysate (whole cell) (ab94112)
Lane 2 : 293T non transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94112 has not yet been referenced specifically in any publications.