Key features and details
- Rabbit polyclonal to IL-15RA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-15RA antibody
See all IL-15RA primary antibodies
DescriptionRabbit polyclonal to IL-15RA
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- Human liver cancer tissue lysate; Human colon cancer tissue lysate; Human fetal kidney tissue lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 28 kDa.|
FunctionReceptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK.
Tissue specificityIsoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
Sequence similaritiesContains 1 Sushi (CCP/SCR) domain.
modificationsA soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
Phosphorylated by activated SYK.
N-glycosylated and O-glycosylated.
Cellular localizationSecreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
- Information by UniProt
- AA690181 antibody
- CD215 antibody
- I15RA_HUMAN antibody
All lanes : Anti-IL-15RA antibody (ab183113) at 1/200 dilution
Lane 1 : Human liver cancer tissue lysate
Lane 2 : Human colon cancer tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat Anti-rabbit HRP at 1/10000 dilution
Predicted band size: 28 kDa
Exposure time: 1 minute
ab183113 has not yet been referenced specifically in any publications.