Recombinant Anti-IL-15RA antibody [EPR24784-142] (ab273022)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)](https://www.abcam.com/ps/products/273/ab273022/Images/ab273022-1-anti-il15raantibody-epr24784142-immunohistochemistry-human-colon.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24784-142] to IL-15RA
- Suitable for: Dot blot, IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-15RA antibody [EPR24784-142]
See all IL-15RA primary antibodies -
Description
Rabbit monoclonal [EPR24784-142] to IL-15RA -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IHC-P, Flow Cytmore details
Unsuitable for: ICC,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon and Human spleen tissues. Flow Cyt: A-172 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24784-142 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt |
1/500.
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| Notes |
|---|
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Dot blot
1/1000. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt
1/500. |
Target
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Function
Receptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK. -
Tissue specificity
Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures. -
Sequence similarities
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsA soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
Phosphorylated by activated SYK.
N-glycosylated and O-glycosylated. -
Cellular localization
Secreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane. - Information by UniProt
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Database links
- Entrez Gene: 3601 Human
- Omim: 601070 Human
- SwissProt: Q13261 Human
- Unigene: 524117 Human
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Alternative names
- AA690181 antibody
- CD215 antibody
- I15RA_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes in human colon (PMID: 23039249).The section was incubated with ab273022 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)](https://www.abcam.com/ps/products/273/ab273022/Images/ab273022-2-anti-il15raantibody-epr24784142-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human spleen.The section was incubated with ab273022 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)](https://www.abcam.com/ps/products/273/ab273022/Images/ab273022-3-anti-il15raantibody-epr24784142-immunohistochemistry-human-skin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human skin (PMID: 30021889). The section was incubated with ab273022 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
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![Flow Cytometry - Anti-IL-15RA antibody [EPR24784-142] (ab273022)](https://www.abcam.com/ps/products/273/ab273022/Images/ab273022-4-anti-il15raantibody-epr24784142-flow-cytometry-daudi.jpg)
Flow Cytometry - Anti-IL-15RA antibody [EPR24784-142] (ab273022)Flow cytometric analysis of Daudi (Human Burkitt's lymphoma lymphoblast, Left)/ A-172(Human brain glioblastoma, Right) cells labelling IL-15RA with ab273022 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Negative control: Daudi (PMID: 8530383). Gated on viable cells.
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![Dot Blot - Anti-IL-15RA antibody [EPR24784-142] (ab273022)](https://www.abcam.com/ps/products/273/ab273022/Images/ab273022-5-anti-il15raantibody-epr24784142-dotblot-human-il15ra-immunogen-recombinant-protein.jpg)
Dot Blot - Anti-IL-15RA antibody [EPR24784-142] (ab273022)Dot blot analysis of IL-15RA using ab273022 at 1/1000 dilution (0.465 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
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![Anti-IL-15RA antibody [EPR24784-142] (ab273022)](https://www.abcam.com/ps/products/273/ab273022/Images/ab273022-6-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-15RA antibody [EPR24784-142] (ab273022)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab273022 has not yet been referenced specifically in any publications.