Key features and details
- Mouse monoclonal [JM7A4] to IL-15RA
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-IL-15RA antibody [JM7A4]
See all IL-15RA primary antibodies
DescriptionMouse monoclonal [JM7A4] to IL-15RA
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IL-15RA.
- LPS and IFN gamma stimulated PBMCs
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesab91270 is affinity purified
Our Abpromise guarantee covers the use of ab91270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 28 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK.
Tissue specificityIsoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
Sequence similaritiesContains 1 Sushi (CCP/SCR) domain.
modificationsA soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
Phosphorylated by activated SYK.
N-glycosylated and O-glycosylated.
Cellular localizationSecreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
- Information by UniProt
- AA690181 antibody
- CD215 antibody
- I15RA_HUMAN antibody
ab91270 staining IL-15RA in normal Human PBMCs by Flow cytometry.
Normal Human PBMCs were isolated, and cultured in standard conditions for 3 hours. Non adherent cells were removed from culture, and remaining cells were stimulated with LPS and IFN-? for 24 hours.
Open/green histogram; stained with 0.25 µg of Purified Mouse IgG2b Isotype Control
Closed/blue histogram; stained with 0.25 µg of ab91270.
Detection utilised Biotin Anti-Mouse IgG and SAv-PE.
Cells in the large scatter population were used for analysis.
ab91270 has been referenced in 3 publications.
- Vidard L et al. CD137 (4-1BB) Engagement Fine-Tunes Synergistic IL-15- and IL-21-Driven NK Cell Proliferation. J Immunol 203:676-685 (2019). PubMed: 31201235
- Vámosi G et al. IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells. Proc Natl Acad Sci U S A 101:11082-7 (2004). PubMed: 15263076
- Dubois S et al. IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells. Immunity 17:537-47 (2002). PubMed: 12433361