Overview

  • Product name

    Anti-IL-15RA antibody [MM0369-5M53]
    See all IL-15RA primary antibodies
  • Description

    Mouse monoclonal [MM0369-5M53] to IL-15RA
  • Host species

    Mouse
  • Specificity

    ab89894 detects IL-15RA.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human IL-15RA.

Properties

Applications

Our Abpromise guarantee covers the use of ab89894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa. WB ab89894 has been tested only against the immunogen and negative results were obtained in ELISA against mouse IL15RA.

Target

  • Function

    Receptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK.
  • Tissue specificity

    Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
  • Sequence similarities

    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications

    A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
    Phosphorylated by activated SYK.
    N-glycosylated and O-glycosylated.
  • Cellular localization

    Secreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA690181 antibody
    • CD215 antibody
    • I15RA_HUMAN antibody
    • IL 15R alpha antibody
    • IL-15 receptor subunit alpha antibody
    • IL-15R-alpha antibody
    • IL-15RA antibody
    • Il15ra antibody
    • Interleukin 15 receptor alpha antibody
    • Interleukin 15 receptor subunit alpha antibody
    • MGC104179 antibody
    • sIL-15 receptor subunit alpha antibody
    • sIL-15R-alpha antibody
    • sIL-15RA antibody
    • Soluble interleukin 15 receptor subunit alpha antibody
    • Soluble interleukin-15 receptor subunit alpha antibody
    see all

References

ab89894 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Recombinant protein (hIL-15ra recombinant protein transduced into EL ce)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Treatment
BME
Specification
hIL-15ra recombinant protein transduced into EL ce
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Gong Yun

Verified customer

Submitted Jan 10 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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