Anti-IL-15RA antibody [RM0103-6C38] (ab86486)
Key features and details
- Rat monoclonal [RM0103-6C38] to IL-15RA
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-IL-15RA antibody [RM0103-6C38]
See all IL-15RA primary antibodies -
Description
Rat monoclonal [RM0103-6C38] to IL-15RA -
Host species
Rat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse IL-15RA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purification notes
The IgG fraction of culture supernatant was purified by protein A/G affinity chromatography and 0.2µm filtered. -
Clonality
Monoclonal -
Clone number
RM0103-6C38 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
Target
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Function
Receptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK. -
Tissue specificity
Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures. -
Sequence similarities
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsA soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
Phosphorylated by activated SYK.
N-glycosylated and O-glycosylated. -
Cellular localization
Secreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane. - Information by UniProt
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Database links
- Entrez Gene: 16169 Mouse
- SwissProt: Q60819 Mouse
- Unigene: 200196 Mouse
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Alternative names
- AA690181 antibody
- CD215 antibody
- I15RA_HUMAN antibody
see all
Datasheets and documents
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Datasheet download
References (2)
ab86486 has been referenced in 2 publications.
- Takashima A et al. Uterine natural killer cells severely decrease in number at gestation day 6 in mice. Biol Reprod 89:101 (2013). WB ; Mouse . PubMed: 24025738
- Chenoweth MJ et al. IL-15 can signal via IL-15Ra, JNK, and NF-?B to drive RANTES production by myeloid cells. J Immunol 188:4149-57 (2012). Blocking ; Mouse . PubMed: 22447977