Anti-IL-16 antibody (ab180792)
Key features and details
- Rabbit polyclonal to IL-16
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IL-16 antibody
See all IL-16 primary antibodies -
Description
Rabbit polyclonal to IL-16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human IL-16 aa 1-305 (N terminal).
Database link: Q14005 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab180792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 142 kDa. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. -
Tissue specificity
Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation. -
Sequence similarities
Contains 4 PDZ (DHR) domains. -
Post-translational
modificationsIsoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. -
Cellular localization
Cytoplasm; Cytoplasm. Nucleus and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3603 Human
- Entrez Gene: 16170 Mouse
- Entrez Gene: 116996 Rat
- Omim: 603035 Human
- SwissProt: Q14005 Human
- SwissProt: O54824 Mouse
- Unigene: 459095 Human
- Unigene: 10137 Mouse
see all -
Alternative names
- HGNC:5980 antibody
- HsT19289 antibody
- IL-16 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IL-16 antibody (ab180792)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab180792. Blue DAPI for nuclear staining.
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Western blot - Anti-IL-16 antibody (ab180792)All lanes : Anti-IL-16 antibody (ab180792) at 1/500 dilution
Lane 1 : A549 cell line extract
Lane 2 : Jurkat cell line extract
Lane 3 : Mouse brain extract
Predicted band size: 142 kDa
Protocols
Datasheets and documents
References (2)
ab180792 has been referenced in 2 publications.
- Yang Z et al. Overexpression of microRNA-34a Attenuates Proliferation and Induces Apoptosis in Pituitary Adenoma Cells via SOX7. Mol Ther Oncolytics 10:40-47 (2018). PubMed: 30109259
- Yang H et al. Diagnostic and prognostic value of serum interleukin-16 in patients with gastric cancer. Mol Med Rep 16:9143-9148 (2017). PubMed: 28990054
