Anti-IL-16 antibody (ab180792)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-IL-16 antibody
See all IL-16 primary antibodies -
Description
Rabbit polyclonal to IL-16 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human IL-16 aa 1-305 (N terminal).
Database link: Q14005 -
Positive control
- A549, Jurkat and Mouse brain lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab180792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/500 - 1/2000. Predicted molecular weight: 142 kDa. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. -
Tissue specificity
Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation. -
Sequence similarities
Contains 4 PDZ (DHR) domains. -
Post-translational
modificationsIsoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. -
Cellular localization
Cytoplasm; Cytoplasm. Nucleus and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3603 Human
- Entrez Gene: 16170 Mouse
- Entrez Gene: 116996 Rat
- Omim: 603035 Human
- SwissProt: Q14005 Human
- SwissProt: O54824 Mouse
- Unigene: 459095 Human
- Unigene: 10137 Mouse
see all -
Alternative names
- HGNC:5980 antibody
- HsT19289 antibody
- IL-16 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IL-16 antibody (ab180792)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab180792. Blue DAPI for nuclear staining. -
Western blot - Anti-IL-16 antibody (ab180792)All lanes : Anti-IL-16 antibody (ab180792) at 1/500 dilution
Lane 1 : A549 cell line extract
Lane 2 : Jurkat cell line extract
Lane 3 : Mouse brain extract
Predicted band size: 142 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yang Z et al. Overexpression of microRNA-34a Attenuates Proliferation and Induces Apoptosis in Pituitary Adenoma Cells via SOX7. Mol Ther Oncolytics 10:40-47 (2018). Read more (PubMed: 30109259) »
