Overview

  • Product name
  • Description
    Rabbit polyclonal to IL-16
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hIL-16 (human Interleukin-16)

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Neutralising
WB
  • Application notes
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.2 ng/ml), a concentration of 0.07 - 0.012 µg/ml of this antibody is required.

    ELISA:To detect hIL-16 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16.

    Western Blot:To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
      Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
      Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
    • Tissue specificity
      Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.
    • Sequence similarities
      Contains 4 PDZ (DHR) domains.
    • Post-translational
      modifications
      Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
    • Cellular localization
      Cytoplasm; Cytoplasm. Nucleus and Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • HGNC:5980 antibody
      • HsT19289 antibody
      • IL-16 antibody
      • IL16 antibody
      • IL16_HUMAN antibody
      • Interleukin 16 precursor antibody
      • Interleukin-16 antibody
      • LCF antibody
      • Lymphocyte chemoattractant factor antibody
      • Neuronal interleukin 16 antibody
      • NIL16 antibody
      • prIL 16 antibody
      • PrIL16 antibody
      • Prointerleukin 16 antibody
      see all

    References

    This product has been referenced in:
    • Ahn DS  et al. Secretion of IL-16 through TNFR1 and calpain-caspase signaling contributes to MRSA pneumonia. Mucosal Immunol N/A:N/A (2014). Read more (PubMed: 24736233) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Hepatocyte)
    Permeabilization
    No
    Specification
    Hepatocyte
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 21 2014

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Gel Running Conditions
    Non-reduced Denaturing (15%)
    Loading amount
    25 µg
    Specification
    primary hepatoctyes
    Blocking step
    I-Block(Applied biosystems) as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jul 09 2013

    Question
    Answer


    The immunogen sequence of ab9563 is:
    MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up