Overview

  • Product name

    Anti-IL-16 antibody [EPR19988]
    See all IL-16 primary antibodies
  • Description

    Rabbit monoclonal [EPR19988] to IL-16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IL-16 aa 1200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14005

  • Positive control

    • WB: H9 and Jurkat whole cell lysates; Human thymus, spleen and tonsil lysates. IHC-P: Human tonsil and colon tissues. ICC/IF: H9 cells. Flow Cyt: H9 cells. IP: H9 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19988
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab207181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 67 kDa.

Observed Mass of target (kDa): 80, 75-40

IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
Flow Cyt 1/600.
IP 1/30.

Target

  • Function

    Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
    Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
    Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
  • Tissue specificity

    Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.
  • Sequence similarities

    Contains 4 PDZ (DHR) domains.
  • Post-translational
    modifications

    Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
  • Cellular localization

    Cytoplasm; Cytoplasm. Nucleus and Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • HGNC:5980 antibody
    • HsT19289 antibody
    • IL-16 antibody
    • IL16 antibody
    • IL16_HUMAN antibody
    • Interleukin 16 precursor antibody
    • Interleukin-16 antibody
    • LCF antibody
    • Lymphocyte chemoattractant factor antibody
    • Neuronal interleukin 16 antibody
    • NIL16 antibody
    • prIL 16 antibody
    • PrIL16 antibody
    • Prointerleukin 16 antibody
    see all

Images

  • All lanes : Anti-IL-16 antibody [EPR19988] (ab207181) at 1/1000 dilution

    Lane 1 : H9 (Human cutaneous T lymphocyte lymphoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa
    Observed band size: 75-40,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The band at 80 kDa is pro-IL-16 and the bands at 40-75 kDa are cleaved fragments. This is consistent with what has been described in the literature (PMID: 15187155, PMID: 9144227, PMID: 9743378, PMID: 14734747).

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-16 with ab207181 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human tonsil is observed [PMID: 10946273]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized H9 (Human cutaneous T lymphocyte lymphoma cell line) cells labeling IL-16 with ab207181 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on H9 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • All lanes : Anti-IL-16 antibody [EPR19988] (ab207181) at 1/1000 dilution

    Lane 1 : Human thymus lysate at 10 µg
    Lane 2 : Human spleen lysate at 10 µg
    Lane 3 : Human tonsil lysate at 20 µg

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa
    Observed band size: 75-40,80 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 5 seconds, Lane 2: 15 seconds, Lane 3: 3 minutes.

    The band at 80 kDa is pro-IL-16 and the bands at 40-75 kDa are cleaved fragments. This is consistent with what has been described in the literature (PMID: 15187155, PMID: 9144227, PMID: 9743378, PMID: 14734747).

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling IL-16 with ab207181 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on stromal cells of human colon is observed [PMID: 11709514]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed H9 (Human cutaneous T lymphocyte lymphoma cell line) cells labeling IL-16 with ab207181 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • IL-16 was immunoprecipitated from 0.35 mg of H9 (Human cutaneous T lymphocyte lymphoma cell line) whole cell lysate with ab207181 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207181 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: H9 whole cell lysate, 10 μg (Input).

    Lane 2: ab207181 IP in H9 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207181 in H9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Note: The band at around 50kDa is a cleaved form of IL-16.

     

References

This product has been referenced in:

  • Huang Y  et al. IL-16 regulates macrophage polarization as a target gene of mir-145-3p. Mol Immunol 107:1-9 (2019). Read more (PubMed: 30634164) »
  • Yang WH  et al. Heterogeneous nuclear ribonucleoprotein M promotes the progression of breast cancer by regulating the axin/ß-catenin signaling pathway. Biomed Pharmacother 105:848-855 (2018). Read more (PubMed: 30021377) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab207181.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up