Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Human IL-17 (internal sequence). ab79056 was raised against a 19 amino acid synthetic peptide from near the center of human IL-17A.
    Database link: Q16552
    (Peptide available as ab190733)

  • Positive control

  • General notes

    The blocking peptide is available as ab190773

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • Cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • Interleukin 17A antibody
    • Interleukin-17A antibody
    • Interleukin17 antibody
    • Interleukin17A antibody
    • OTTHUMP00000016597 antibody
    • OTTMUSP00000046003 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse thymus tissue staining IL-17 with ab79056 at 20µg/ml

  • Paraffin-embedded human tonsil tissue stained for IL-17 using ab79056 at 5 µg/ml in immunohistochemical analysis.

  • Paraffin embedded mouse colon tissue staining IL-17 using ab79056 at 2μg/ml by Immunohistochemistry. 

  • Anti-IL-17 antibody (ab79056) at 1 µg/ml + Recombinant human IL-17 at 0.001 µg

    Predicted band size: 18 kDa

  • ab79056 staining IL-17 in Dog lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween-20 (0.05%) and blocked with 10% serum for 2 hours at 24°C; antigen retrieval was by heat mediation with citrate buffer (0.01M). Samples were incubated with primary antibody (1/100 in PBS +10% serum) for 14 hours at 4°C. A Biotin-conjugated Horse pan-specific anti-IgG polyclonal (1/20) was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-IL-17 antibody (ab79056) at 0.5 µg/ml
    Lane 2 : Anti-IL-17 antibody (ab79056) at 1 µg/ml

    All lanes : A-20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa
    Additional bands at: 22 kDa, 28 kDa, 32 kDa. We are unsure as to the identity of these extra bands.



    The higher molecular weight bands seen in the Western blot may represent glycosylated IL-17.

  • All lanes : Anti-IL-17 antibody (ab79056)

    Lane 1 : human thymus tissue lysate at 1 µg/ml
    Lane 2 : human thymus tissue lysate at 2 µg/ml

    Predicted band size: 18 kDa

References

This product has been referenced in:

  • Ito JT  et al. Th17/Treg imbalance in COPD progression: A temporal analysis using a CS-induced model. PLoS One 14:e0209351 (2019). Read more (PubMed: 30629626) »
  • Hui Z  et al. Visfatin promotes the malignancy of human acute myeloid leukemia cells via regulation of IL-17. Eur J Pharmacol 853:103-110 (2019). Read more (PubMed: 30876976) »
See all 54 Publications for this product

Customer reviews and Q&As

1-10 of 34 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse tumor tissue)
Permeabilization
Yes - PBST(0.5% v/v triton X-100, sigma)
Specification
mouse tumor tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 04 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
100 ng/ml LPS
Specification
mouse hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 21 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formain

Abcam user community

Verified customer

Submitted May 08 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted May 08 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat
Permeabilization
No
Specification
Colon
Blocking step
Serum as blocking agent for 8 minute(s) · Concentration: 25% · Temperature: 37°C
Fixative
Glutaraldehyde

Ms. Maria Daskalaki

Verified customer

Submitted Nov 13 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: microwave with citrate buffer pH 6.0
Permeabilization
No
Specification
Mammary gland
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Natalia Salvetti

Verified customer

Submitted Oct 09 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (salivary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Tris pH=9.0 / 1mM EDTA
Permeabilization
No
Specification
salivary gland
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 09 2013

Answer

Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any IL17 antibody tested in the species and application you are using should be suitable for your applications.

Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

Regarding a negative control, we can suggest mouse skeletal muscle or heart as potential negative controls for IL17.

I hope this will be helpful. Should you have any further questions please do not hesitate to ask.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver whole extract)
Gel Running Conditions
Reduced Denaturing (14% Tris-glycine gel)
Loading amount
100 µg
Specification
Liver whole extract
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Sep 22 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 10 2012

1-10 of 34 Abreviews or Q&A

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