Anti-IL-17 antibody (ab79056)
- Datasheet
- References (54)
- Protocols
Overview
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Product name
Anti-IL-17 antibody
See all IL-17 primary antibodies -
Description
Rabbit polyclonal to IL-17 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Dog, Human -
Immunogen
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Positive control
- Purchase matching WB positive control:Recombinant Human IL-17 protein
- WB: A-20 cell lysate; Human thymus tissue lysate. IHC-P: Human tonsil tissue; Dog lymph node tissue.
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General notes
The blocking peptide is available as ab190773
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab79056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080). -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3605 Human
- Entrez Gene: 16171 Mouse
- Omim: 603149 Human
- SwissProt: Q16552 Human
- SwissProt: Q62386 Mouse
- Unigene: 41724 Human
- Unigene: 5419 Mouse
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Alternative names
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17 antibody (ab79056)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse thymus tissue staining IL-17 with ab79056 at 20µg/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17 antibody (ab79056)Paraffin-embedded human tonsil tissue stained for IL-17 using ab79056 at 5 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17 antibody (ab79056)Paraffin embedded mouse colon tissue staining IL-17 using ab79056 at 2μg/ml by Immunohistochemistry.
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Western blot - Anti-IL-17 antibody (ab79056)Anti-IL-17 antibody (ab79056) at 1 µg/ml + Recombinant human IL-17 at 0.001 µg
Predicted band size: 18 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17 antibody (ab79056)This image is courtesy of an Abreview submitted by Claudia Zizzadoroab79056 staining IL-17 in Dog lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween-20 (0.05%) and blocked with 10% serum for 2 hours at 24°C; antigen retrieval was by heat mediation with citrate buffer (0.01M). Samples were incubated with primary antibody (1/100 in PBS +10% serum) for 14 hours at 4°C. A Biotin-conjugated Horse pan-specific anti-IgG polyclonal (1/20) was used as the secondary antibody.
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Western blot - Anti-IL-17 antibody (ab79056)Lane 1 : Anti-IL-17 antibody (ab79056) at 0.5 µg/ml
Lane 2 : Anti-IL-17 antibody (ab79056) at 1 µg/ml
All lanes : A-20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 22 kDa, 28 kDa, 32 kDa. We are unsure as to the identity of these extra bands.The higher molecular weight bands seen in the Western blot may represent glycosylated IL-17.
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Western blot - Anti-IL-17 antibody (ab79056)All lanes : Anti-IL-17 antibody (ab79056)
Lane 1 : human thymus tissue lysate at 1 µg/ml
Lane 2 : human thymus tissue lysate at 2 µg/ml
Predicted band size: 18 kDa
Protocols
References
This product has been referenced in:
- Ito JT et al. Th17/Treg imbalance in COPD progression: A temporal analysis using a CS-induced model. PLoS One 14:e0209351 (2019). Read more (PubMed: 30629626) »
- Hui Z et al. Visfatin promotes the malignancy of human acute myeloid leukemia cells via regulation of IL-17. Eur J Pharmacol 853:103-110 (2019). Read more (PubMed: 30876976) »
