Anti-IL-17A antibody [4K5F6] (ab189377)
Key features and details
- Mouse monoclonal [4K5F6] to IL-17A
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-IL-17A antibody [4K5F6]
See all IL-17A primary antibodies -
Description
Mouse monoclonal [4K5F6] to IL-17A -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human, Recombinant fragment
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human IL-17A aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_002181.1).
Database link: Q16552 -
Positive control
- IHC-P: Human small intestine tissue. Flow Cyt (Intra): Human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs. WB: Human full-length recombinant IL-17A protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4K5F6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa.
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use 0.1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa. |
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use 0.1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3605 Human
- Omim: 603149 Human
- SwissProt: Q16552 Human
- Unigene: 41724 Human
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Alternative names
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labelling IL-17A with ab189377 at 10 µg/ml.
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Intracellular flow cytometric analysis of IL-17A in Human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, labeling IL-17A with ab189377 at 0.1 ug/106 cells (red) Green represents isotype control antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
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All lanes : Anti-IL-17A antibody [4K5F6] (ab189377) at 0.2 µg/ml
Lane 1 : Human full-length recombinant IL-17A protein
Lane 2 : Mouse full-length recombinant IL-17A protein
Lane 3 : Rat full-length recombinant IL-17A protein
Lysates/proteins at 0.1 µg/ml per lane.
Predicted band size: 15 kDa
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab189377 has been referenced in 7 publications.
- Catar RA et al. Angiogenic Role of Mesothelium-Derived Chemokine CXCL1 During Unfavorable Peritoneal Tissue Remodeling in Patients Receiving Peritoneal Dialysis as Renal Replacement Therapy. Front Immunol 13:821681 (2022). PubMed: 35185912
- Wang T et al. Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35457188
- Agak GW et al. Extracellular traps released by antimicrobial TH17 cells contribute to host defense. J Clin Invest 131:N/A (2021). PubMed: 33211671
- Liu B et al. Reciprocal regulation of interleukin-17A and interleukin-22 secretion through aryl hydrocarbon receptor activation in CD4+ T cells of patients with vitiligo. Exp Ther Med 21:158 (2021). PubMed: 33456525
- Cheng R et al. Expression of IL17A in endometrial carcinoma and effects of IL17A on biological behaviour in Ishikawa cells. J Int Med Res 48:300060520950563 (2020). PubMed: 32878530
- Zhong W et al. Increased expression of IRF8 in tumor cells inhibits the generation of Th17 cells and predicts unfavorable survival of diffuse large B cell lymphoma patients. Oncotarget 8:49757-49772 (2017). PubMed: 28537908
- Huang Q et al. Interleukin-17A-Induced Epithelial-Mesenchymal Transition of Human Intrahepatic Biliary Epithelial Cells: Implications for Primary Biliary Cirrhosis. Tohoku J Exp Med 240:269-275 (2016). PubMed: 27916760