Key features and details
- Mouse monoclonal [4K5F6] to IL-17A
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-IL-17A antibody [4K5F6]
See all IL-17A primary antibodies
DescriptionMouse monoclonal [4K5F6] to IL-17A
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
- Human small intestine tissue; Human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs; Human full-length recombinant IL-17A protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab189377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa.|
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use 0.1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionInduces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
Tissue specificityRestricted to activated memory T-cells.
Sequence similaritiesBelongs to the IL-17 family.
modificationsFound both in glycosylated and nonglycosylated forms.
- Information by UniProt
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labelling IL-17A with ab189377 at 10 µg/ml.
All lanes : Anti-IL-17A antibody [4K5F6] (ab189377) at 0.2 µg/ml
Lane 1 : Human full-length recombinant IL-17A protein
Lane 2 : Mouse full-length recombinant IL-17A protein
Lane 3 : Rat full-length recombinant IL-17A protein
Lysates/proteins at 0.1 µg/ml per lane.
Predicted band size: 15 kDa
Intracellular flow cytometric analysis of IL-17A in Human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, labeling IL-17A with ab189377 at 0.1 ug/106 cells (red) Green represents isotype control antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
ab189377 has been referenced in 1 publication.
- Zhong W et al. Increased expression of IRF8 in tumor cells inhibits the generation of Th17 cells and predicts unfavorable survival of diffuse large B cell lymphoma patients. Oncotarget 8:49757-49772 (2017). PubMed: 28537908