Key features and details
- Rabbit polyclonal to IL-17A
- Suitable for: WB, IHC-P, Neutralising, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-17A antibody
See all IL-17A primary antibodies
DescriptionRabbit polyclonal to IL-17A
Tested applicationsSuitable for: WB, IHC-P, Neutralising, Sandwich ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IL-17A.
- Human T-lymphocyte tissue
FormLyophilized:Add 50 µl Sterile water
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Constituent: 100% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab136668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 18 kDa. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Neutralising||Use a concentration of 0.9 - 1.3 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of Human IL17A (6.00 ng/ml), a concentration of 0.1-0.18 ug/ml of this antibody is required.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. ab136668 should be used as a capture antibody.|
FunctionInduces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
Tissue specificityRestricted to activated memory T-cells.
Sequence similaritiesBelongs to the IL-17 family.
modificationsFound both in glycosylated and nonglycosylated forms.
- Information by UniProt
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
ab136668 has been referenced in 10 publications.
- Chen J et al. Long non-coding RNA-HOTAIR promotes the progression of sepsis by acting as a sponge of miR-211 to induce IL-6R expression. Exp Ther Med 18:3959-3967 (2019). PubMed: 31656541
- Wang J et al. Cervical carcinoma high-expressed long non-coding RNA 1 may promote growth of colon adenocarcinoma through interleukin-17A. Oncol Lett 18:1491-1496 (2019). PubMed: 31423215
- Zhao PX et al. IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease. Int J Ophthalmol 11:36-42 (2018). PubMed: 29375988
- Tamassia N et al. A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It. Front Immunol 9:795 (2018). PubMed: 29719541
- Tamassia N et al. Cytokine production by human neutrophils: Revisiting the "dark side of the moon". Eur J Clin Invest N/A:e12952 (2018). PubMed: 29772063
- Kang Y et al. Activation of the TLR4/MyD88 signaling pathway contributes to the development of human hepatocellular carcinoma via upregulation of IL-23 and IL-17A. Oncol Lett 15:9647-9654 (2018). PubMed: 29928340
- Cai S et al. IFN-? induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense. Mucosal Immunol 9:718-29 (2016). PubMed: 26349661
- Alunno A et al. Mobilization of lymphatic endothelial precursor cells and lymphatic neovascularization in primary Sjögren's syndrome. J Cell Mol Med 20:613-22 (2016). PubMed: 26828975
- Arterbery AS et al. Production of Proinflammatory Cytokines by Monocytes in Liver-Transplanted Recipients with De Novo Autoimmune Hepatitis Is Enhanced and Induces TH1-like Regulatory T Cells. J Immunol 196:4040-51 (2016). PubMed: 27183637
- Gao Y et al. Circulating FoxP3+ Regulatory T and Interleukin17-Producing Th17 Cells Actively Influence HBV Clearance in De Novo Hepatitis B Virus Infected Patients after Orthotopic Liver Transplantation. PLoS One 10:e0137881 (2015). PubMed: 26367459