Overview

  • Product name

  • Description

    Rabbit polyclonal to IL-17A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Neutralising, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IL-17A.

  • Positive control

    • Human T-lymphocyte tissue

Properties

  • Form

    Lyophilised:Add 50 µl Sterile water
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 18 kDa. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Neutralising Use a concentration of 0.9 - 1.3 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of Human IL17A (6.00 ng/ml), a concentration of 0.1-0.18 ug/ml of this antibody is required.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. ab136668 should be used as a capture antibody.

Target

  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • interleukin 17A antibody
    • Interleukin-17A antibody
    • OTTHUMP00000016597 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human T-lymphocyte tissue labelling IL-17A with ab136668 at 5µg/ml after heat-induced antigen retrieval.

References

This product has been referenced in:

  • Zhao PX  et al. IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease. Int J Ophthalmol 11:36-42 (2018). Read more (PubMed: 29375988) »
  • Tamassia N  et al. A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It. Front Immunol 9:795 (2018). Read more (PubMed: 29719541) »
See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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